Anti-n-Myc antibody [NCM II 100] - ChIP Grade (ab16898)


  • Product name
    Anti-n-Myc antibody [NCM II 100] - ChIP Grade
    See all n-Myc primary antibodies
  • Description
    Mouse Monoclonal [NCM II 100] to n-Myc - ChIP Grade
  • Host species
  • Specificity
    This antibody reacts with N-myc encoded proteins and their cleavage products.
  • Tested applications
    Suitable for: IHC-Fr, IP, WB, ICC/IF, Flow Cyt, ChIPmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein corresponding to Human n-Myc. Recombinant fusion protein. Original clone reference: PubMed ID - 2426708.
    Database link: P04198

  • Positive control
    • IMR5 Cells
  • General notes

    It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used.




Our Abpromise guarantee covers the use of ab16898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 - 5 µg/ml.

See references

IP Use a concentration of 1 - 2 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64, 67 kDa).

See references

ICC/IF Use a concentration of 1 - 5 µg/ml.

Pubmed ID: 2426708

Flow Cyt Use 0.5µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ChIP Use at an assay dependent concentration. PubMed: 19495417Suggested to be used at 2µg.


  • Function
    May function as a transcription factor.
  • Involvement in disease
    Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
    Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
    Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
  • Sequence similarities
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage
    Expressed during fetal development.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • bHLHe37 antibody
    • Class E basic helix-loop-helix protein 37 antibody
    • MODED antibody
    • mycn antibody
    • MYCN_HUMAN antibody
    • N myc antibody
    • N myc proto oncogene protein antibody
    • N-myc proto-oncogene protein antibody
    • Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibody
    • Neuroblastoma MYC oncogene antibody
    • NMYC antibody
    • NMYC proto oncogene protein antibody
    • ODED antibody
    • Oncogene NMYC antibody
    • pp65/67 antibody
    • V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody
    • v myc avian myelocytomatosis viral related oncogene neuroblastoma derived antibody
    • v myc myelocytomatosis viral related oncogene neuroblastoma derived antibody
    see all


  • Immunoprecipitation of N-Myc from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2).
    ab16898 used at 1 mg/sample.
  • Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.


This product has been referenced in:
  • Gupta S  et al. Reversible LSD1 inhibition with HCI-2509 induces the p53 gene expression signature and disrupts the MYCN signature in high-risk neuroblastoma cells. Oncotarget 9:9907-9924 (2018). Read more (PubMed: 29515779) »
  • Agarwal S  et al. MYCN acts as a direct co-regulator of p53 in MYCN amplified neuroblastoma. Oncotarget 9:20323-20338 (2018). Read more (PubMed: 29755654) »

See all 24 Publications for this product

Customer reviews and Q&As

Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (NuPage Bis-Tris 4-12% gel)
Mouse Cell lysate - whole cell (cerebellar granule neuron precursors)
cerebellar granule neuron precursors
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 26 2013

What species is your lysate from?

For human samples, the n-myc oncoprotein has a predicted MW of 50 kDa (, but runs in WB at around 64 kDa. Cleavage products would run at smaller MWs.

Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Neural tube)
Neural tube
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate/Citrate+NaBH4/Tris-EDTA (ph=9)/10% SDS/ NaBH4/10% SDS + NaBH4
Yes - 0.5% Triton
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Mar 19 2010

Thank you for your enquiry. According to the following publication, monoclonal ab16898 n-Myc antibody [clone NCM II 100] interacts with an epitope unique to an N-myc region not present in C-myc regions tested (3' 60% mouse C-myc coding region and 5'...

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Thank you for your enquiry. This antibody has been characterised with others raised against NMYC in the following paper which may be of help to you: Identification and characterisation of the NMYC gene product in human neuroblastoma cells by monocl...

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Thank you for contacting us for technical support. The problem you are experiencing may be due to the fact that the antibody is very fragile. We recommend to aliquot and store at -20°C upon arrival. Can you tell me how you proceeded? If you can also t...

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Thank you for your researcher's further information. The information that accompanies this antibody is that the buffer is comprised of no preservatives as detailed below: Preservative: None Constituents: 50% Glycerol, 0.05M Sodium phosphate. Furt...

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We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purc...

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Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-formalin fixed paraffin embedded sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, p...

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