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Fusion protein corresponding to Human n-Myc. Recombinant fusion protein.
Original clone reference: PubMed ID - 2426708.
Database link: P04198
It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used.
Our Abpromise guarantee covers the use of ab16898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use a concentration of 1 - 5 µg/ml.
|IP||Use a concentration of 1 - 2 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64, 67 kDa).
|ICC/IF||Use a concentration of 1 - 5 µg/ml.
Pubmed ID: 2426708
|Flow Cyt||Use 0.5µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|ChIP||Use at an assay dependent concentration. PubMed: 19495417Suggested to be used at 2µg.|
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