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Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human N myc interactor aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q13287
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Our Abpromise guarantee covers the use of ab170870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 35 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: N myc interactor knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: K562 cell lysate (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab170870 observed at 39 kDa. Red - loading control, ab18058, observed at 124 kDa.
ab170870 was shown to recognize N myc interactor when N myc interactor knockout samples were used, along with additional cross-reactive bands. Wild-type and N myc interactor knockout samples were subjected to SDS-PAGE. Ab170870 and ab18058 (loading control to Vinculin) were diluted at 1/500 and 1/10000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Flow cytometric analysis of permeabilized K562 cells labeling N myc interactor with ab170870 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).
ab170870 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"