Key features and details
- Rabbit polyclonal to N myc interactor/NMI
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-N myc interactor/NMI antibody
See all N myc interactor/NMI primary antibodies
DescriptionRabbit polyclonal to N myc interactor/NMI
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
- WB: Rat heart lysate. IHC-P: Human colon cancer tissue. ICC/IF: HepG2 cells.
This product was previously labelled as N myc interactor
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
Our Abpromise guarantee covers the use of ab224675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 35 kDa).|
RelevanceNMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT mediated transcription in response to cytokines IL2 and IFN gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.
- N myc (and STAT) interactor antibody
- N myc and STAT interactor antibody
- N myc interactor antibody
Anti-N myc interactor/NMI antibody (ab224675) at 1/500 dilution + Rat heart lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 35 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Paraffin-embedded human colon cancer tissue stained for N myc interactor/NMI using ab224675 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for N myc interactor/NMI (green) using ab224675 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224675 has not yet been referenced specifically in any publications.