Key features and details
- Rabbit polyclonal to n-Myc/MYCN
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-n-Myc/MYCN antibody
See all n-Myc/MYCN primary antibodies
DescriptionRabbit polyclonal to n-Myc/MYCN
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human n-Myc/MYCN aa 410-464 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: P04198
- MCF7 and SK-N-SH cell lysates;Human brain and Human colon carcinoma tissues.
This product was previously labelled as n-Myc
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab217873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500.|
|WB||1/100 - 1/1000. Predicted molecular weight: 50 kDa.|
FunctionMay function as a transcription factor.
Involvement in diseaseNote=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Developmental stageExpressed during fetal development.
- Information by UniProt
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling n-Myc/MYCN with ab217873 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue labeling n-Myc/MYCN with ab217873 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
All lanes : Anti-n-Myc/MYCN antibody (ab217873) at 1/200 dilution
Lane 1 : MCF7 cell lysates
Lane 2 : SK-N-SH cell lysates
All lanes : secondary antibody at 1/10000 dilution
Predicted band size: 50 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab217873 has not yet been referenced specifically in any publications.