Overview

  • Product name

  • Description

    Rabbit polyclonal to n-Myc/MYCN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Non human primates
  • Immunogen

    Synthetic peptide corresponding to Human n-Myc/MYCN aa 1-100 conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human n-Myc/MYCN.

    Read Abcam's proprietary immunogen policy (Peptide available as ab31595.)
    Database link: P04198
    (Peptide available as ab31595, ab31596)

  • Positive control

    • Recombinant Human n-Myc/MYCN protein (ab114325) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Heart (Human) Whole Cell Lysate - fetal normal tissue Heart (Mouse) Tissue Lysate Skeletal Muscle (Mouse) Tissue Lysate
  • General notes

     This product was previously labelled as n-Myc

     

Properties

Applications

Our Abpromise guarantee covers the use of ab24193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 22216302

Target

  • Function

    May function as a transcription factor.
  • Involvement in disease

    Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
    Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
    Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage

    Expressed during fetal development.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bHLHe37 antibody
    • Class E basic helix-loop-helix protein 37 antibody
    • MODED antibody
    • mycn antibody
    • MYCN_HUMAN antibody
    • N myc antibody
    • N myc proto oncogene protein antibody
    • N-myc proto-oncogene protein antibody
    • Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibody
    • Neuroblastoma MYC oncogene antibody
    • NMYC antibody
    • NMYC proto oncogene protein antibody
    • ODED antibody
    • Oncogene NMYC antibody
    • pp65/67 antibody
    • V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody
    • v myc avian myelocytomatosis viral related oncogene neuroblastoma derived antibody
    • v myc myelocytomatosis viral related oncogene neuroblastoma derived antibody
    see all

Images

  • All lanes : Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/ml

    Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue
    Lane 2 : Heart (Mouse) Tissue Lysate
    Lane 3 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG VHH Single Domain (HRP) (ab191866) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa
    Additional bands at: 190 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab24193 overnight at 4°C. Antibody binding was detected using an anti-rabbit IgG VHH single domain antibody conjugated to HRP (ab191866), and visualised using ECL development solution ab133406.

  • All lanes : Anti-n-Myc/MYCN antibody (ab24193) at 1 µg/ml

    Lane 1 : Heart (Human) Whole Cell Lysate - fetal normal tissue (ab29432)
    Lane 2 : Heart (Human) Nuclear Lysate - fetal normal tissue (ab29428)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 49 kDa
    Observed band size: 49 kDa
    Additional bands at: 25 kDa (possible cleavage fragment), 25 kDa (possible cross reactivity), 33 kDa (possible cleavage fragment), 33 kDa (possible cross reactivity), 39 kDa (possible cleavage fragment), 39 kDa (possible cross reactivity)

References

This product has been referenced in:

  • Wang XH  et al. FGF represses metastasis of neuroblastoma regulated by MYCN and TGF-ß1 induced LMO1 via control of let-7 expression. Brain Res 1704:219-228 (2019). Read more (PubMed: 30321496) »
  • Zhu S  et al. BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation. Exp Ther Med 17:3883-3890 (2019). Read more (PubMed: 31007733) »
See all 8 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (2-week-old mouse cerebellum)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
2-week-old mouse cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 29 2015

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Kidney
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Dec 30 2010

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