Anti-n-Myc/MYCN antibody (ab272918)
Key features and details
- Rabbit polyclonal to n-Myc/MYCN
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-n-Myc/MYCN antibody
See all n-Myc/MYCN primary antibodies -
Description
Rabbit polyclonal to n-Myc/MYCN -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human n-Myc/MYCN (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: P04198 -
Positive control
- WB: Neuro2A, C8D30, NIH/3T3, Raw 264.7 and C2C12 whole cell extract. U-87 MG, SK-N-SH, IMR32 SK-N-AS whole cell extract. IRM32 nuclear extract. Mouse and rat brain tissue extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272918 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000.
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| Notes |
|---|
|
WB
1/500 - 1/3000. |
Target
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Function
May function as a transcription factor. -
Involvement in disease
Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585]. -
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain. -
Developmental stage
Expressed during fetal development. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4613 Human
- Entrez Gene: 18109 Mouse
- Entrez Gene: 298894 Rat
- Omim: 164840 Human
- SwissProt: P04198 Human
- SwissProt: P03966 Mouse
- SwissProt: Q63379 Rat
- Unigene: 25960 Human
see all -
Alternative names
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
see all
Images
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Western blot - Anti-n-Myc/MYCN antibody (ab272918)All lanes : Anti-n-Myc/MYCN antibody (ab272918) at 1/1000 dilution
Lane 1 : Neuro2A whole cell extract
Lane 2 : C8D30 whole cell extract
Lane 3 : NIH/3T3 whole cell extract
Lane 4 : Raw 264.7 whole cell extract
Lane 5 : C2C12 whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-n-Myc/MYCN antibody (ab272918)All lanes : Anti-n-Myc/MYCN antibody (ab272918) at 1/1000 dilution
Lane 1 : U-87 MG whole cell extract
Lane 2 : SK-N-SH whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
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Western blot - Anti-n-Myc/MYCN antibody (ab272918)All lanes : Anti-n-Myc/MYCN antibody (ab272918) at 1/2500 dilution
Lane 1 : IRM32 whole cell extract
Lane 2 : IRM32 nuclear extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody -
Western blot - Anti-n-Myc/MYCN antibody (ab272918)All lanes : Anti-n-Myc/MYCN antibody (ab272918) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody10% SDS-PAGE
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab272918 has not yet been referenced specifically in any publications.