For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    n-mycmycn-antibody-ab272918.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Transcription Domain Families HLH / Leucine Zipper
Share by email

Anti-n-Myc/MYCN antibody (ab272918)

  • Datasheet
  • SDS
Submit a review Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-n-Myc/MYCN antibody (ab272918)
  • Western blot - Anti-n-Myc/MYCN antibody (ab272918)
  • Western blot - Anti-n-Myc/MYCN antibody (ab272918)
  • Western blot - Anti-n-Myc/MYCN antibody (ab272918)

Key features and details

  • Rabbit polyclonal to n-Myc/MYCN
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

You may also be interested in

Peptide
Human c-Myc peptide (ab166837)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-c-Myc antibody [Y69] (ab32072)

View more associated products

Overview

  • Product name

    Anti-n-Myc/MYCN antibody
    See all n-Myc/MYCN primary antibodies
  • Description

    Rabbit polyclonal to n-Myc/MYCN
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human n-Myc/MYCN (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: P04198

  • Positive control

    • WB: Neuro2A, C8D30, NIH/3T3, Raw 264.7 and C2C12 whole cell extract. U-87 MG, SK-N-SH, IMR32 SK-N-AS whole cell extract. IRM32 nuclear extract. Mouse and rat brain tissue extract.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH / Leucine Zipper
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Transcription factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Positive Controls

    • Mouse brain tissue lysate - total protein (ab30151)
    • NIH/3T3 whole cell lysate (ab7179)
    • RAW 264.7 whole cell lysate (ab7187)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000.
Notes
WB
1/500 - 1/3000.

Target

  • Function

    May function as a transcription factor.
  • Involvement in disease

    Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
    Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
    Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage

    Expressed during fetal development.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P04198 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4613 Human
    • Entrez Gene: 18109 Mouse
    • Entrez Gene: 298894 Rat
    • Omim: 164840 Human
    • SwissProt: P04198 Human
    • SwissProt: P03966 Mouse
    • SwissProt: Q63379 Rat
    • Unigene: 25960 Human
    • Unigene: 16469 Mouse
    • Unigene: 81116 Rat
    see all
  • Alternative names

    • bHLHe37 antibody
    • Class E basic helix-loop-helix protein 37 antibody
    • MODED antibody
    • mycn antibody
    • MYCN_HUMAN antibody
    • N myc antibody
    • N myc proto oncogene protein antibody
    • N-myc proto-oncogene protein antibody
    • Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibody
    • Neuroblastoma MYC oncogene antibody
    • NMYC antibody
    • NMYC proto oncogene protein antibody
    • ODED antibody
    • Oncogene NMYC antibody
    • pp65/67 antibody
    • V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody
    • v myc avian myelocytomatosis viral related oncogene neuroblastoma derived antibody
    • v myc myelocytomatosis viral related oncogene neuroblastoma derived antibody
    see all

Images

  • Western blot - Anti-n-Myc/MYCN antibody (ab272918)
    Western blot - Anti-n-Myc/MYCN antibody (ab272918)
    All lanes : Anti-n-Myc/MYCN antibody (ab272918) at 1/1000 dilution

    Lane 1 : Neuro2A whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 whole cell extract
    Lane 4 : Raw 264.7 whole cell extract
    Lane 5 : C2C12 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    10% SDS-PAGE

  • Western blot - Anti-n-Myc/MYCN antibody (ab272918)
    Western blot - Anti-n-Myc/MYCN antibody (ab272918)
    All lanes : Anti-n-Myc/MYCN antibody (ab272918) at 1/1000 dilution

    Lane 1 : U-87 MG whole cell extract
    Lane 2 : SK-N-SH whole cell extract
    Lane 3 : IMR32 whole cell extract
    Lane 4 : SK-N-AS whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    10% SDS-PAGE

  • Western blot - Anti-n-Myc/MYCN antibody (ab272918)
    Western blot - Anti-n-Myc/MYCN antibody (ab272918)
    All lanes : Anti-n-Myc/MYCN antibody (ab272918) at 1/2500 dilution

    Lane 1 : IRM32 whole cell extract
    Lane 2 : IRM32 nuclear extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody
  • Western blot - Anti-n-Myc/MYCN antibody (ab272918)
    Western blot - Anti-n-Myc/MYCN antibody (ab272918)
    All lanes : Anti-n-Myc/MYCN antibody (ab272918) at 1/1000 dilution

    Lane 1 : Mouse brain tissue extract
    Lane 2 : Rat brain tissue extract

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody


    10% SDS-PAGE

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab272918? Please let us know so that we can cite the reference in this datasheet.

ab272918 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

There are currently no Customer reviews or Questions for ab272918.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.