Product nameAnti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade
See all n-Myc/MYCN primary antibodies
DescriptionRabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade
Tested applicationsSuitable for: ChIP, WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human n-Myc/MYCN aa 1-300. The exact sequence is proprietary.
Database link: P04198
- WB: IMR-32 whole cell lysate. IP: IMR-32 whole cell lysate. ChIP: Chromatin prepared from IMR-32 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
Our Abpromise guarantee covers the use of ab227822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use 5 µg for 25 µg of chromatin.|
|WB||1/1000. Detects a band of approximately 49-62 kDa (predicted molecular weight: 50 kDa).|
FunctionMay function as a transcription factor.
Involvement in diseaseNote=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Developmental stageExpressed during fetal development.
- Information by UniProt
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
Chromatin was prepared from IMR-32 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab227822 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). Primers and probes are located in the first kb of the transcribed region.
All lanes : Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822) at 1/1000 dilution
Lane 1 : IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 103 seconds
Blocking and dilution buffer: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 11034201; PMID: 27197171; PMID: 23792191).
Negative control: HeLa (PMID: 27197171).
n-Myc/MYCN was immunoprecipitated from 0.35 mg of IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate with ab227822 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227822 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: IMR-32 whole cell lysate 10 μg (Input).
Lane 2: ab227822 IP in IMR-32 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227822 in IMR-32 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 17938259; PMID: 2657399).
ab227822 has not yet been referenced specifically in any publications.