Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade
- Suitable for: ChIP, WB, IP
- Reacts with: Human
Product nameAnti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade
See all n-Myc/MYCN primary antibodies
DescriptionRabbit monoclonal [EPR18982-8R-3] to n-Myc/MYCN - ChIP Grade
Tested applicationsSuitable for: ChIP, WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human n-Myc/MYCN aa 1-300. The exact sequence is proprietary.
Database link: P04198
- WB: IMR-32 whole cell lysate. IP: IMR-32 whole cell lysate. ChIP: Chromatin prepared from IMR-32 cells.
This product was previously labelled as n-Myc
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 49% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityProtein A purified
- Epigenetics and Nuclear Signaling
- Domain Families
- HLH / Leucine Zipper
- HLH / Leucine Zipper
Our Abpromise guarantee covers the use of ab227822 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use 5 µg for 25 µg of chromatin.|
|WB||1/1000. Detects a band of approximately 49-62 kDa (predicted molecular weight: 50 kDa).|
FunctionMay function as a transcription factor.
Involvement in diseaseNote=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Developmental stageExpressed during fetal development.
- Information by UniProt
- bHLHe37 antibody
- Class E basic helix-loop-helix protein 37 antibody
- MODED antibody
Chromatin was prepared from IMR-32 cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10min. The ChIP was performed with 25 µg of chromatin, 5 µg of ab227822 (red), and 20 µl of Protein A/G sepharose beads. 5 μg of rabbit normal IgG was added to the beads control (gray). The immunoprecipitated DNA was quantified by real time PCR (SYBR green approach). Primers and probes are located in the first kb of the transcribed region.
All lanes : Anti-n-Myc/MYCN antibody [EPR18982-8R-3] - ChIP Grade (ab227822) at 1/1000 dilution
Lane 1 : IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Exposure time: 103 seconds
Blocking and dilution buffer: 5% NFDM/TBST
The expression profile observed is consistent with what has been described in the literature (PMID: 11034201; PMID: 27197171; PMID: 23792191).
Negative control: HeLa (PMID: 27197171).
n-Myc/MYCN was immunoprecipitated from 0.35 mg of IMR-32 (human neuroblastoma neuroblast cell line) whole cell lysate with ab227822 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227822 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: IMR-32 whole cell lysate 10 μg (Input).
Lane 2: ab227822 IP in IMR-32 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227822 in IMR-32 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The expression profile observed is consistent with what has been described in the literature (PMID: 17938259; PMID: 2657399).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227822 has not yet been referenced specifically in any publications.