Application
Western blot
Sample
Mouse Cell lysate - whole cell (cerebellar granule neuron precursors)
Gel Running Conditions
Reduced Denaturing (NuPage Bis-Tris 4-12% gel)
Loading amount
20 µg
Specification
cerebellar granule neuron precursors
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/100
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Jackson 115-035-003 anti-mouse IgG (H+L) HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55 kDa Non-specific: 80 kDa
Additional data
Additional Notes
Cultivated cells in medium with sonic hedgehog lysed with RIPA PVDF membrane. Both lanes are the same cell type and treatment, just two different batches of cells.
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Submitted Nov 26 2013