Anti-n-Myc/MYCN antibody [NCM II 100] - ChIP Grade (ab16898)

Mouse monoclonal n-Myc antibody [NCM II 100]. Validated in WB, IP, IHC, Flow Cyt, ChIP, ICC/IF and tested in Mouse, Human. Cited in 25 publication(s). Independently reviewed in 2 review(s).

Overview

  • Product name

    Anti-n-Myc/MYCN antibody [NCM II 100] - ChIP Grade
    See all n-Myc/MYCN primary antibodies
  • Description

    Mouse monoclonal [NCM II 100] to n-Myc/MYCN - ChIP Grade
  • Host species

    Mouse
  • Specificity

    This antibody reacts with N-myc/MYCN encoded proteins and their cleavage products.

  • Tested applications

    Suitable for: IHC-Fr, IP, WB, ICC/IF, Flow Cyt, ChIPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Fusion protein corresponding to Human n-Myc/MYCN. Recombinant fusion protein. Original clone reference: PubMed ID - 2426708.
    Database link: P04198

  • Positive control

    • IMR5 Cells
  • General notes

    It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used.

     

     This product was previously labelled as n-Myc

     This product was changed from ascites to tissue culture supernatant on 17 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Applications

Our Abpromise guarantee covers the use of ab16898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.

See references

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 64, 67 kDa).

See references

ICC/IF Use at an assay dependent concentration.

Pubmed ID: 2426708

Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ChIP Use at an assay dependent concentration. PubMed: 19495417

Suggested to be used at 2µg.

Target

  • Function

    May function as a transcription factor.
  • Involvement in disease

    Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
    Defects in MYCN are the cause of microcephaly-oculo-digito-esophageal-duodenal syndrome (MODED) [MIM:164280]; also known as oculodigitoesophagoduodenal syndrome (ODED). Microcephaly-oculo-digito-esophageal-duodenal syndrome is characterized by variable combinations of esophageal and duodenal atresias, microcephaly, learning disability and limb malformations. Cardiac and renal malformations, vertebral anomalies, and deafness have also been described.
    Defects in MYCN are the cause of microcephaly and digital abnormalities with normal intelligence (MCPHDANI) [MIM:602585].
  • Sequence similarities

    Contains 1 basic helix-loop-helix (bHLH) domain.
  • Developmental stage

    Expressed during fetal development.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • bHLHe37 antibody
    • Class E basic helix-loop-helix protein 37 antibody
    • MODED antibody
    • mycn antibody
    • MYCN_HUMAN antibody
    • N myc antibody
    • N myc proto oncogene protein antibody
    • N-myc proto-oncogene protein antibody
    • Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene antibody
    • Neuroblastoma MYC oncogene antibody
    • NMYC antibody
    • NMYC proto oncogene protein antibody
    • ODED antibody
    • Oncogene NMYC antibody
    • pp65/67 antibody
    • V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog antibody
    • v myc avian myelocytomatosis viral related oncogene neuroblastoma derived antibody
    • v myc myelocytomatosis viral related oncogene neuroblastoma derived antibody
    see all

Images

  • Immunoprecipitation of N-Myc/MYCN from S35 methionine-labeled IMR-5 cells using ab16898 (lane 1) and Normal Mouse IgG (lane 2).
    ab16898 used at 1 mg/sample.

    This image was generated using the ascites version of the product.

  • Overlay histogram showing SH-SY5Y cells stained with ab16898 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16898, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Sun Q  et al. SEMA6D regulates perinatal cardiomyocyte proliferation and maturation in mice. Dev Biol 452:1-7 (2019). Read more (PubMed: 31042497) »
  • Gupta S  et al. Reversible LSD1 inhibition with HCI-2509 induces the p53 gene expression signature and disrupts the MYCN signature in high-risk neuroblastoma cells. Oncotarget 9:9907-9924 (2018). Read more (PubMed: 29515779) »
See all 26 Publications for this product

Customer reviews and Q&As

1-9 of 9 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (cerebellar granule neuron precursors)
Gel Running Conditions
Reduced Denaturing (NuPage Bis-Tris 4-12% gel)
Loading amount
20 µg
Specification
cerebellar granule neuron precursors
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 26 2013

Answer


What species is your lysate from?


For human samples, the n-myc oncoprotein has a predicted MW of 50 kDa (http://www.uniprot.org/uniprot/P04198), but runs in WB at around 64 kDa. Cleavage products would run at smaller MWs.

Read More
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Neural tube)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate/Citrate+NaBH4/Tris-EDTA (ph=9)/10% SDS/ NaBH4/10% SDS + NaBH4
Permeabilization
Yes - 0.5% Triton
Specification
Neural tube
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 19 2010

Answer

Thank you for your enquiry. According to the following publication, monoclonal ab16898 n-Myc antibody [clone NCM II 100] interacts with an epitope unique to an N-myc region not present in C-myc regions tested (3' 60% mouse C-myc coding region and 5' 30% C-myc coding region); please see the second paragraph on page 5930 of the publication. I hope this is helpful. Please do not hesitate to contact me if you require any further assistance.

Read More

Answer

Thank you for your enquiry. This antibody has been characterised with others raised against NMYC in the following paper which may be of help to you: Identification and characterisation of the NMYC gene product in human neuroblastoma cells by monoclonal antibodies with defined specificities. Ikegaki N et al. Prodedings of National Acadamy of Science USA. 83 p5929. This indiates that epitope for this antibody lies in the second exon of NMYC and it will recognise the cleavage products from NMYC. I hope this helps. Please get in touch again if you require any more advice. With thanks

Read More

Answer

Thank you for contacting us for technical support. The problem you are experiencing may be due to the fact that the antibody is very fragile. We recommend to aliquot and store at -20°C upon arrival. Can you tell me how you proceeded? If you can also tell me your order details I will look at the shipping details to investigate if the antibody suffered from a delay. I look forward to hearing from you to resolve this matter,

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Answer

Thank you for your researcher's further information. The information that accompanies this antibody is that the buffer is comprised of no preservatives as detailed below: Preservative: None Constituents: 50% Glycerol, 0.05M Sodium phosphate. Furthermore we recommend that the antibody is stored at -20°C and that freeze / thaw cycles are avoided. Again as stated below: Storage instructions: Aliquot and store at -20°C. Avoid freeze / thaw cycles. We DO recommend that the antibody is diluted in PBS for certain applications prior to use. However, this certainly would NOT be recommended if the intention is to store the antibody in the fridge for two weeks, especially given the lack of preservative. It is therefore of no surprise to me that the antibody has developed mould ("contaminated"). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will offer you 50 Abcam Points which can be redeemed on a number of rewards (a further 100 points will be offered for an image).

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Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in IHC-formalin fixed paraffin embedded sections. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on by submitting an Abreview and in return we will award you 50 Abcam Points, which can be redeemed on a number of rewards (a further 100 Abcam Points will be offered for an image). If you have any additional questions, please contact us again.

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