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Thank you for your time yesterday discussing the antibody problem . The researcher informed us that the Abcam antibody 16898 was diluted as recommended with PBS, and was stored at 4 degrees. After about two weeks a yellow deposit has appeared and the fluid is cloudy. According to the user, the instructions that came with the antibody clearly said not to add preservatives. This is the first antibody they use that got contaminated.
Asked on Mar 17 2006
Thank you for your researcher's further information. The information that accompanies this antibody is that the buffer is comprised of no preservatives as detailed below: Preservative: None Constituents: 50% Glycerol, 0.05M Sodium phosphate. Furthermore we recommend that the antibody is stored at -20°C and that freeze / thaw cycles are avoided. Again as stated below: Storage instructions: Aliquot and store at -20°C. Avoid freeze / thaw cycles. We DO recommend that the antibody is diluted in PBS for certain applications prior to use. However, this certainly would NOT be recommended if the intention is to store the antibody in the fridge for two weeks, especially given the lack of preservative. It is therefore of no surprise to me that the antibody has developed mould ("contaminated"). I hope this information helps, please do not hesitate to contact us if you need any more advice or information.
Answered on Mar 20 2006