Product nameN-Ras Activation Assay Kit
Sample typeTissue, Adherent cells, Suspension cells
Species reactivityReacts with: Mouse, Rat, Human
N-Ras Activation Assay Kit (ab211160) uses Raf1 RBD Agarose beads to selectively isolate and pull-down the active form of Ras from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Ras is detected by western blot analysis using an Anti-N-Ras specific polyclonal antibody, which reacts with the human, mouse and rat protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
Ras, a 21 kD protein, regulates a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, Ras regulates molecular events by cycling between an inactive GDP-bound form and an active GTP-bound form. In its active GTP-bound state, Ras binds specifically to the Ras-binding domain (RBD)of Raf1 to control downstream signaling cascades. Since defects in Ras signaling may result in malignant transformation, the activation of Ras proteins is tightly controlled in normal cells.
The 3 Ras genes in human are H-Ras, N-Ras and K-Ras – it is estimated now that approximately 20% – 25% of all human tumors have activating mutations in one of the Ras genes.
N-Ras (Neuroblastoma Ras viral oncogene homolog) was the third Ras gene to be discovered (after H-Ras and K-Ras). Mutations on this gene have been associated with somatic rectal cancer, follicular thyroid cancer, Noonan syndrome and juvenile myelomonocytic leukemia.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 20 tests 5 tests ab211176 - Raf-1 RBD Agarose Beads 1 x 800µl 1 x 200µl 100X GTPyS 1 x 50µl 1 x 20µl 100X GDP 1 x 50µl 1 x 20µl 5X Assay/Lysis Buffer 1 x 30ml 4 x 2ml Anti-N-Ras Rabbit polyclonal antibody 1 x 40µl 1 x 10µl N-Ras Immunoblot Positive Control 1 x 100µl 1 x 100µl
FunctionRas proteins bind GDP/GTP and possess intrinsic GTPase activity.
Involvement in diseaseDefects in NRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia.
Defects in NRAS are the cause of Noonan syndrome type 6 (NS6) [MIM:613224]. A syndrome characterized by facial dysmorphic features such as hypertelorism, a downward eyeslant and low-set posteriorly rotated ears. Other features can include short stature, a short neck with webbing or redundancy of skin, cardiac anomalies, deafness, motor delay and variable intellectual deficits.
Sequence similaritiesBelongs to the small GTPase superfamily. Ras family.
modificationsPalmitoylated by the ZDHHC9-GOLGA7 complex. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.
Cellular localizationCell membrane. Golgi apparatus membrane. Shuttles between the plasma membrane and the Golgi apparatus.
- Information by UniProt
- GTPase NRas
ab211160 has not yet been referenced specifically in any publications.