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    n-terminal-propeptide-of-procollagen-type-iii-piiinp-antibody-epr24257-178-bsa-and-azide-ab285003.pdf

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RecombinantRabMAb

Recombinant Anti-N-terminal propeptide of procollagen type III (PIIINP) antibody [EPR24257-178] - BSA and Azide (ab285003)

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Sandwich ELISA - Anti-N-terminal propeptide of procollagen type III (PIIINP) antibody - BSA and Azide free (Capture) (ab285003)

    Key features and details

    • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
    • Rabbit monoclonal [EPR24257-178] to N-terminal propeptide of procollagen type III (PIIINP) - BSA and Azide free (Capture)
    • Suitable for: Sandwich ELISA
    • Reacts with: Mouse

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    Overview

    • Product name

      Anti-N-terminal propeptide of procollagen type III (PIIINP) antibody [EPR24257-178] - BSA and Azide
      See all N-terminal propeptide of procollagen type III (PIIINP) primary antibodies
    • Description

      Rabbit monoclonal [EPR24257-178] to N-terminal propeptide of procollagen type III (PIIINP) - BSA and Azide free (Capture)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: Sandwich ELISAmore details
    • Species reactivity

      Reacts with: Mouse
    • General notes

      ab285003 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Collagen III. The recommended pair for sandwich ELISA is:
           Capture: ab285003, Mouse N-terminal propeptide of procollagen type III (PIIINP) Capture Antibody (unconjugated)
           Detector: ab285109, Mouse N-terminal propeptide of procollagen type III (PIIINP) Detector Antibody (unconjugated)
      The reference range value is 31.25 - 2000 pg/mL.

      The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

      Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free production
      For more information see here.

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C.
    • Storage buffer

      Constituent: 100% PBS
    • Carrier free

      Yes
    • Concentration information loading...
    • Clonality

      Monoclonal
    • Clone number

      EPR24257-178
    • Isotype

      IgG

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
      • Goat Anti-Rabbit IgG H&L (Biotin) (ab207995)
      • Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)
    • Conjugation kits

      • Alkaline phosphatase Conjugation Kit - Lightning-Link® (ab102850)
      • HRP Conjugation Kit - Lightning-Link® (ab102890)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
    • sELISA pair antibody

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • Streptavidin Conjugation Kit - Lightning-Link® (ab102921)
      • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
      • Anti-N-terminal propeptide of procollagen type III (PIIINP) antibody [EPR24257-88] - BSA and Azide f (ab285109)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab285003 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24257-88] to N-terminal propeptide of procollagen type III (PIIINP) - BSA and Azide free (Detector) (ab285109).
    Notes
    Sandwich ELISA
    Use at an assay dependent concentration. Can be paired for Sandwich ELISA with PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918) and Streptavidin Conjugation Kit - Lightning-Link® (ab102921) and Biotinylation Kit / Biotin Conjugation Kit (Fast and Type A) - Lightning-Link® (ab201795) and Rabbit monoclonal [EPR24257-88] to N-terminal propeptide of procollagen type III (PIIINP) - BSA and Azide free (Detector) (ab285109).

    Images

    • Sandwich ELISA - Anti-N-terminal propeptide of procollagen type III (PIIINP) antibody - BSA and Azide free (Capture) (ab285003)
      Sandwich ELISA - Anti-N-terminal propeptide of procollagen type III (PIIINP) antibody - BSA and Azide free (Capture) (ab285003)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab285003? Please let us know so that we can cite the reference in this datasheet.

    ab285003 has not yet been referenced specifically in any publications.

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