Key features and details
- Rabbit polyclonal to N WASP
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-N WASP antibody
See all N WASP primary antibodies
DescriptionRabbit polyclonal to N WASP
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human N WASP (N terminal). N-terminal 250AA
Database link: O00401
- OVCAR3, BT474, SW480 PC3 and MCF7 cell extracts; mouse brain and kidney extracts.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab187527 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 55 kDa.|
FunctionRegulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
Sequence similaritiesContains 1 CRIB domain.
Contains 1 WH1 domain.
Contains 2 WH2 domains.
Cellular localizationCytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.
- Information by UniProt
- DKFZp779G0847 antibody
- MGC48327 antibody
- N-WASP antibody
All lanes : Anti-N WASP antibody (ab187527) at 1/500 dilution
Lane 1 : OVCAR3 cell extract
Lane 2 : BT474 cell extract
Lane 3 : SW480 cell extract
Lane 4 : PC3 cell extract
Lane 5 : MCF7 cell extract
Lane 6 : mouse brain extract
Lane 7 : mouse kidney extract
Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling N WASP with ab187527 at 1/100. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue labelling N WASP with ab187527 at 1/100. Magnification: 400x.
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab187527. Blue DAPI for nuclear staining.
ab187527 has been referenced in 1 publication.
- Leduc C & Etienne-Manneville S Regulation of microtubule-associated motors drives intermediate filament network polarization. J Cell Biol 216:1689-1703 (2017). PubMed: 28432079