Overview

  • Product name
    Anti-N WASP (phospho Y256) antibody
    See all N WASP primary antibodies
  • Description
    Rabbit polyclonal to N WASP (phospho Y256)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Phospho N WASP (Tyr 256) synthetic peptide (coupled to carrier protein) corresponding to amino acid residues around tyrosine 256 of human N WASP. The human WASP sequence has a similar peptide sequence surrounding tyrosine 291.

  • Positive control
    • Pervanadate treated A431, HeLa, Jurkat and endothelial cells for SDS PAGE immunoblots.

Properties

Applications

Our Abpromise guarantee covers the use of ab23395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa). The antibody detects a 63 or 65 kDa protein corresponding to phosphorylated N WASP on SDS PAGE immunoblots of A431, HeLa, Jurkat and endothelial cells treated with pervanadate. Weak bands are also observed at higher molecular weights after pervanadte treatment which may be due to low crossreactivity with phosphotyrosine.
ELISA 1/20000.

Target

  • Function
    Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
  • Sequence similarities
    Contains 1 CRIB domain.
    Contains 1 WH1 domain.
    Contains 2 WH2 domains.
  • Cellular localization
    Cytoplasm > cytoskeleton. Nucleus. Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp779G0847 antibody
    • MGC48327 antibody
    • N-WASP antibody
    • Neural Wiskott Aldrich syndrome protein antibody
    • Neural Wiskott-Aldrich syndrome protein antibody
    • NWASP antibody
    • Wasl antibody
    • WASL_HUMAN antibody
    • WASPB antibody
    • Wiskott Aldrich syndrome gene like antibody
    • Wiskott Aldrich syndrome gene like protein antibody
    • Wiskott Aldrich syndrome like antibody
    • WiskottAldrich syndrome like antibody
    see all

Images

  • All lanes : Anti-N WASP (phospho Y256) antibody (ab23395) at 1/10000 dilution

    Lane 1 : Control A431 cells 20ug/lane
    Lane 2 : Pervanadate-treated A431 cells 20 ug/lane

    Predicted band size: 55 kDa



    Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.

References

This product has been referenced in:
  • Zhang W  et al. Rho kinase collaborates with p21-activated kinase to regulate actin polymerization and contraction in airway smooth muscle. J Physiol 596:3617-3635 (2018). Read more (PubMed: 29746010) »
  • Zhang W  et al. p21-Activated kinase (Pak) regulates airway smooth muscle contraction by regulating paxillin complexes that mediate actin polymerization. J Physiol 594:4879-900 (2016). Read more (PubMed: 27038336) »
See all 5 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. ab23395 was raised using a phospho-N-WASP (Tyr-256) synthetic peptide (coupled to BSA) corresponding to amino acid residues around tyrosine 256 of human N-WASP. The human WASP sequence has a two amino acid difference in the same region surrounding tyrosine 291. It is possible that the antibody will recognize Tyr-291 in WASP; however this reactivity has so far not yet been shown. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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