Product nameAnti-N4BP1 antibody
See all N4BP1 primary antibodies
DescriptionRabbit polyclonal to N4BP1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Gorilla, Orangutan
- HeLa, Jurkat and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab197079 was affinity purified using an epitope specific to N4BP1 immobilized on solid support
Our Abpromise guarantee covers the use of ab197079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 100 kDa.|
FunctionInhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
Tissue specificityDetected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
Sequence similaritiesBelongs to the N4BP1 family.
modificationsMonoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
- Information by UniProt
- FLJ31821 antibody
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All lanes : Anti-N4BP1 antibody (ab197079) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 100 kDa
Exposure time: 3 minutes
Lysates prepared with NETN lysis buffer.
Detection of N4BP1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197079 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab197079 has not yet been referenced specifically in any publications.