Recombinant Anti-N4BP1 antibody [EPNCIR118] (ab133610)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR118] to N4BP1
- Suitable for: WB, Flow Cyt (Intra)
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-N4BP1 antibody [EPNCIR118]
See all N4BP1 primary antibodies -
Description
Rabbit monoclonal [EPNCIR118] to N4BP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment within Mouse N4BP1 aa 250-500. The exact sequence is proprietary.
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Positive control
- F9, Mouse brain, B16 F0, and PC12 cell lysates; F9 cells
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General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Michael Kuehn. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Human: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR118 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
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Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 100 kDa. |
Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. -
Tissue specificity
Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary. -
Sequence similarities
Belongs to the N4BP1 family. -
Post-translational
modificationsMonoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. - Information by UniProt
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Database links
- Entrez Gene: 80750 Mouse
- Entrez Gene: 291921 Rat
- SwissProt: Q6A037 Mouse
- Unigene: 25117 Mouse
- Unigene: 428374 Mouse
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Alternative names
- FLJ31821 antibody
- KIAA0615 antibody
- MGC176730 antibody
see all
Images
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All lanes : Anti-N4BP1 antibody [EPNCIR118] (ab133610) at 1/1000 dilution
Lane 1 : F9 cell lysate
Lane 2 : Mouse brain cell lysate
Lane 3 : B16 F0 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 100 kDa -
Intracellular flow cytometrical analysis of N4BP1 in permeabilized F9 cells, using ab133610 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab133610 has not yet been referenced specifically in any publications.