Overview

  • Product name

    Anti-N4BP1 antibody [EPNCIR118]
    See all N4BP1 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR118] to N4BP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse N4BP1 aa 250-500. The exact sequence is proprietary.

  • Positive control

    • F9, Mouse brain, B16 F0, and PC12 cell lysates; F9 cells
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Michael Kuehn. View antibodies from NCI Center for Cancer Research Collaboration.

    Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR118
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133610 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 100 kDa.
IP 1/10 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
    • Tissue specificity

      Detected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
    • Sequence similarities

      Belongs to the N4BP1 family.
    • Post-translational
      modifications

      Monoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization

      Nucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
    • Information by UniProt
    • Database links

    • Alternative names

      • FLJ31821 antibody
      • KIAA0615 antibody
      • MGC176730 antibody
      • N4BP1 antibody
      • N4BP1_HUMAN antibody
      • NEDD4 binding protein 1 antibody
      • NEDD4-binding protein 1 antibody
      see all

    Images

    • All lanes : Anti-N4BP1 antibody [EPNCIR118] (ab133610) at 1/1000 dilution

      Lane 1 : F9 cell lysate
      Lane 2 : Mouse brain cell lysate
      Lane 3 : B16 F0 cell lysate
      Lane 4 : PC12 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 100 kDa

    • Flow Cytometrical analysis of N4BP1 in permeabilized F9 cells, using ab133610 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).

    References

    ab133610 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133610.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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