Product nameAnti-N4BP1 antibody [EPNCIR118]
See all N4BP1 primary antibodies
DescriptionRabbit monoclonal [EPNCIR118] to N4BP1
Tested applicationsSuitable for: WB, IP, Flow Cytmore details
Unsuitable for: ICC or IHC-P
Species reactivityReacts with: Mouse, Rat
Recombinant fragment within Mouse N4BP1 aa 250-500. The exact sequence is proprietary.
- F9, Mouse brain, B16 F0, and PC12 cell lysates; F9 cells
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Michael Kuehn. View antibodies from NCI Center for Cancer Research Collaboration.
Human: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 100 kDa.|
|IP||1/10 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates.
Tissue specificityDetected in heart, lung, brain, liver, skeletal muscle, pancreas, kidney, spleen, testis and ovary.
Sequence similaritiesBelongs to the N4BP1 family.
modificationsMonoubiquitinated by NEDD4. Polyubiquitinated, leading to its degradation by the proteasome. Sumoylated by SUMO1, abrogating polyubiquitination and subsequent degradation. Desumoylated by SENP1, leading to accumulation in PML nuclear bodies.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus > nucleolus. Nucleus > PML body. Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated.
- Information by UniProt
- FLJ31821 antibody
- KIAA0615 antibody
- MGC176730 antibody
All lanes : Anti-N4BP1 antibody [EPNCIR118] (ab133610) at 1/1000 dilution
Lane 1 : F9 cell lysate
Lane 2 : Mouse brain cell lysate
Lane 3 : B16 F0 cell lysate
Lane 4 : PC12 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 100 kDa
Flow Cytometrical analysis of N4BP1 in permeabilized F9 cells, using ab133610 at a dilution of 1/100 (red) or a rabbit IgG (negative) (green).
ab133610 has not yet been referenced specifically in any publications.