Overview

  • Product name

    Anti-N6-methyladenosine (m6A) antibody [17-3-4-1]
    See all N6-methyladenosine (m6A) primary antibodies
  • Description

    Mouse monoclonal [17-3-4-1] to N6-methyladenosine (m6A)
  • Host species

    Mouse
  • Specificity

    This antibody recognises N6-methyladenosine in both modified RNA and DNA.
  • Tested applications

    Suitable for: Northwestern, Dot blot, IP, Southern Blotmore details
  • Species reactivity

    Reacts with: Mouse, Human, Saccharomyces cerevisiae
  • Immunogen

    Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to bovine serum albumin. Hapten N6-methyladenosine-5'-mono-phosphate conjugated to BSA of all N6-methyladenosine

  • Positive control

    • methylated RNA (negative control non methylated RNA); single stranded DNA;

Properties

Applications

Our Abpromise guarantee covers the use of ab208577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Northwestern Use at an assay dependent concentration.
Dot blot 1/100.

optimal working dilution needs to be determined by the end user.

IP Use at an assay dependent concentration.

Best results are obtained by pre-binding the antibody to the beads before washing off unbound immunoglobulin. It is recommended to start with an excess of antibody in order to ensure the Protein G beads are saturated.Used 3µg in 500µl for IP which yielded positive results.

Southern Blot Use at an assay dependent concentration.

Target

  • Relevance

    N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
  • Alternative names

    • m6A antibody
    • N6-methyladenosine antibody

Images

  • N6-methyladenosine (m6A) was immunoprecipitated using ab208577 from radiolabeled synthetic methylated transcript (Bound); radiolabeled Non methylated control (Non bound) and radiolabeled Input RNA control.

     

     

  • Use of ab208577 for northwestern blot using synthetic methlyated and non methlyated RNA.

  • Lane 1 Single stranded DNA

    Lane 2 Double stranded DNA

    Labelled with ab208577. Arrow shows m6A containing fragment.

References

This product has been referenced in:

See all 2 Publications for this product

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