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    n6-methyladenosine-m6a-antibody-ab151230.pdf

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Anti-N6-methyladenosine (m6A) antibody (ab151230)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (59)

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Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (ab151230)

    Key features and details

    • Rabbit polyclonal to N6-methyladenosine (m6A)
    • Suitable for: Nucleotide Array
    • Reacts with: Species independent
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free (ab190886)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-N6-methyladenosine (m6A) antibody
      See all N6-methyladenosine (m6A) primary antibodies
    • Description

      Rabbit polyclonal to N6-methyladenosine (m6A)
    • Host species

      Rabbit
    • Tested applications

      Suitable for: Nucleotide Arraymore details
    • Species reactivity

      Reacts with: Species independent
    • Immunogen

      Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to keyhole limpet haemocyanin.

    • Positive control

      • ab151230 was tested on a oligonucleotide array against RNA oligomers; N6-methyladenosine (m6A) and unmodified adenosine. 2Ome-RNA oligomers carry a methyl group at the 2’-OH residue of the ribose molecule, making them resistant to most ribonucleases.
    • General notes

      N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.

      The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Constituent: PBS

      PBS prepared using DEPC-treated water.
      The use of RNase inhibitor is recommended.
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Metabolism
      • Types of disease
      • Obesity
      • Epigenetics and Nuclear Signaling
      • RNA
      • RNA / Nucleotides

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab151230 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Nucleotide Array
    Use a concentration of 2 µg/ml.
    Notes
    Nucleotide Array
    Use a concentration of 2 µg/ml.

    Target

    • Relevance

      N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
    • Alternative names

      • m6A antibody
      • N6-methyladenosine antibody

    Images

    • Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (ab151230)
      Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (ab151230)

      All batches of ab151230 are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.

      2Ome-m6A - N6-methyladenosine

      2Ome-m6A_unmod - unmodified adenosine

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (59)

    Publishing research using ab151230? Please let us know so that we can cite the reference in this datasheet.

    ab151230 has been referenced in 59 publications.

    • You A  et al. TTC22 promotes m6A-mediated WTAP expression and colon cancer metastasis in an RPL4 binding-dependent pattern. Oncogene N/A:N/A (2022). WB ; Human . PubMed: 35798874
    • Holmes MJ  et al. m6A RNA methylation facilitates pre-mRNA 3'-end formation and is essential for viability of Toxoplasma gondii. PLoS Pathog 17:e1009335 (2021). PubMed: 34324585
    • Han T  et al. Identification of a robust signature for clinical outcomes and immunotherapy response in gastric cancer: based on N6-methyladenosine related long noncoding RNAs. Cancer Cell Int 21:432 (2021). PubMed: 34399770
    • Roberts JT  et al. Identification of m6A residues at single-nucleotide resolution using eCLIP and an accessible custom analysis pipeline. RNA 27:527-541 (2021). PubMed: 33376190
    • Tassinari V  et al. ADAR1 is a new target of METTL3 and plays a pro-oncogenic role in glioblastoma by an editing-independent mechanism. Genome Biol 22:51 (2021). PubMed: 33509238
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-4 of 4 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-N6-methyladenosine (m6A) antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MCF10A expressing Lamin B1-DAM fusion protein)
    Permeabilization
    No
    Specification
    MCF10A expressing Lamin B1-DAM fusion protein
    Blocking step
    FBS as blocking agent for 5 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    70% Ethanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 07 2017

    RIP abreview for Anti-N6-methyladenosine (m6A) antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    RIP
    Sample
    Human Cell lysate - other (Purified mRNA from HeLa cell)
    Negative control
    None.
    Immuno-precipitation step
    Other - Protein A Dynabeads
    Specification
    Purified mRNA from HeLa cell
    Detection step
    Real-time PCR
    Type
    RIP without cross-linking
    Duration of cross-linking step: 0 second(s)
    Positive control
    A positive group using known m6A-Ab for cross-comparison.
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Boxuan Zhao

    Verified customer

    Submitted Jan 05 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-N6-methyladenosine (m6A) antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Adherent cell types)
    Permeabilization
    Yes - PBS + 0.25% Triton X100
    Specification
    Adherent cell types
    Blocking step
    BlockAid from Invitrogen as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 01 2014

    Question

    How was the nucleotide array performed? Was the antibody fluorescently tagged or were the oligomers fluorescently tagged?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 14 2014

    Answer

    The nucleotide array was performed using 2'-O-methyl-modified ribonucleotides, printed on to nitrocellulose-coated slides (the 2'-O-methyl-modification makes the oligos resistant to most ribonucleases). This array was then probed with the primary antibody (ab151230), which was then detected using a fluorescently labelled anti-Rabbit secondary.

    Read More

    Elena Valued Customer

    Abcam Scientific Support

    Answered on Jul 14 2014

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