Anti-N6-methyladenosine (m6A) antibody (ab151230)
Key features and details
- Rabbit polyclonal to N6-methyladenosine (m6A)
- Suitable for: Nucleotide Array
- Reacts with: Mouse, Human, Drosophila melanogaster
- Isotype: IgG
Overview
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Product name
Anti-N6-methyladenosine (m6A) antibody
See all N6-methyladenosine (m6A) primary antibodies -
Description
Rabbit polyclonal to N6-methyladenosine (m6A) -
Host species
Rabbit -
Tested applications
Suitable for: Nucleotide Arraymore details -
Species reactivity
Reacts with: Mouse, Human, Drosophila melanogaster
Predicted to work with: Rat, a wide range of other species
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Immunogen
Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to keyhole limpet haemocyanin.
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Positive control
- ab151230 was tested on a oligonucleotide array against RNA oligomers; N6-methyladenosine (m6A) and unmodified adenosine. 2Ome-RNA oligomers carry a methyl group at the 2’-OH residue of the ribose molecule, making them resistant to most ribonucleases.
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General notes
N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.
The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituent: PBS
PBS prepared using DEPC-treated water.
The use of RNase inhibitor is recommended. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab151230 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Nucleotide Array | Use a concentration of 2 µg/ml. |
Target
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Relevance
N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment. -
Alternative names
- m6A antibody
- N6-methyladenosine antibody
Images
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Nucleotide Array - Anti-N6-methyladenosine (m6A) antibody (ab151230)All batches of ab151230 are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.
2Ome-m6A - N6-methyladenosine
2Ome-m6A_unmod - unmodified adenosine
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (25)
ab151230 has been referenced in 25 publications.
- Zhang H et al. Dynamic landscape and evolution of m6A methylation in human. Nucleic Acids Res 48:6251-6264 (2020). PubMed: 32406913
- Song T et al. Zfp217 mediates m6A mRNA methylation to orchestrate transcriptional and post-transcriptional regulation to promote adipogenic differentiation. Nucleic Acids Res N/A:N/A (2019). PubMed: 31037292
- Liu XM et al. Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates. Nat Chem Biol 15:865-871 (2019). PubMed: 31383972
- Cheng Y et al. m6A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment. Cell Rep 28:1703-1716.e6 (2019). PubMed: 31412241
- Jia R et al. m6A modification suppresses ocular melanoma through modulating HINT2 mRNA translation. Mol Cancer 18:161 (2019). PubMed: 31722709
