Anti-N6-methyladenosine (m6A) antibody (ab151230)

Rabbit polyclonal N6-methyladenosine (m6A) antibody. Validated in Nucleotide Array and tested in Mouse, Human, Drosophila melanogaster. Cited in 12 publication(s).

Overview

  • Product name

    Anti-N6-methyladenosine (m6A) antibody
    See all N6-methyladenosine (m6A) primary antibodies
  • Description

    Rabbit polyclonal to N6-methyladenosine (m6A)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Nucleotide Arraymore details
  • Species reactivity

    Reacts with: Mouse, Human, Drosophila melanogaster
    Predicted to work with: Rat, a wide range of other species
  • Immunogen

    Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • ab151230 was tested on a oligonucleotide array against RNA oligomers; N6-methyladenosine (m6A) and unmodified adenosine. 2Ome-RNA oligomers carry a methyl group at the 2’-OH residue of the ribose molecule, making them resistant to most ribonucleases.
  • General notes

    N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis.

    The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Constituent: PBS

    PBS prepared using DEPC-treated water.
    The use of RNase inhibitor is recommended.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab151230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Nucleotide Array Use a concentration of 2 µg/ml.

Target

  • Relevance

    N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
  • Alternative names

    • m6A antibody
    • N6-methyladenosine antibody

Images

  • All batches of ab151230 are tested in Nucleotide Array against N6-methyladenosine (m6A) and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.

    2Ome-m6A - N6-methyladenosine

    2Ome-m6A_unmod - unmodified adenosine

References

This product has been referenced in:

  • Liu L  et al. Differential m6A methylomes between two major life stages allows potential regulations in Trypanosoma brucei. Biochem Biophys Res Commun 508:1286-1290 (2019). Read more (PubMed: 30573362) »
  • Hawley BR & Jaffrey SR Transcriptome-Wide Mapping of m6 A and m6 Am at Single-Nucleotide Resolution Using miCLIP. Curr Protoc Mol Biol 126:e88 (2019). Read more (PubMed: 30874375) »
See all 18 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF10A expressing Lamin B1-DAM fusion protein)
Permeabilization
No
Specification
MCF10A expressing Lamin B1-DAM fusion protein
Blocking step
FBS as blocking agent for 5 minute(s) · Concentration: 2% · Temperature: RT°C
Fixative
70% Ethanol

Abcam user community

Verified customer

Submitted Feb 07 2017

Application
RIP
Sample
Human Cell lysate - other (Purified mRNA from HeLa cell)
Negative control
None.
Immuno-precipitation step
Other - Protein A Dynabeads
Specification
Purified mRNA from HeLa cell
Detection step
Real-time PCR
Type
RIP without cross-linking
Duration of cross-linking step: 0 second(s)
Positive control
A positive group using known m6A-Ab for cross-comparison.

Dr. Boxuan Zhao

Verified customer

Submitted Jan 05 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Adherent cell types)
Permeabilization
Yes - PBS + 0.25% Triton X100
Specification
Adherent cell types
Blocking step
BlockAid from Invitrogen as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 01 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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