Overview

  • Product name

    Anti-N6-methyladenosine (m6A) antibody
    See all N6-methyladenosine (m6A) primary antibodies
  • Description

    Rabbit polyclonal to N6-methyladenosine (m6A)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blotmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Other Immunogen Type. (N6-methyladenosine (m6A) conjugated to LPH).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.05% Sodium azide, 0.05% Proclin
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab232905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/400.

Target

  • Relevance

    N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
  • Alternative names

    • m6A antibody
    • N6-methyladenosine antibody

Images

  • To demonstrate the specificity of ab232905 against N6-methyladenosine (m6A), a Dot Blot analysis was performed using synthetic oligonucleotides containing different modified bases. 150 and 50 pmol of the oligonucleotides were spotted on the membrane. The antibody was diluted 1/400 in PBS-T containing 10 % skimmed milk and 1% BSA. ab232905 shows a high specificity for the oligonucleotide with the N6-methyladenosine modification.

References

ab232905 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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