Recombinant
RabMAb

Recombinant Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free (ab236160)

Overview

  • Product name

    Anti-N6-methyladenosine (m6A) antibody [mAbcam 190886] - BSA and Azide free
    See all N6-methyladenosine (m6A) primary antibodies
  • Description

    Rabbit monoclonal [mAbcam 190886] to N6-methyladenosine (m6A) - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Nucleotide Arraymore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Chemical/ Small Molecule corresponding to N6-methyladenosine (m6A) conjugated to keyhole limpet haemocyanin.

  • Positive control

    • Tested on a oligonucleotide array against RNA oligomers; N6-methyladenosine (m6A) and unmodified adenosine.
  • General notes

    ab236160 is the carrier-free version of ab190886 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab236160 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    N6-methyladenosine (m6A) is a post-transcriptional modification of RNA. m6A modification has been identified in all classes of RNA (rRNA, tRNA and mRNA) and is catalysed by an evolutionary conserved multi-subunit enzyme, methyltransferase like 3 (METTL3). Cellular and viral RNA has been known to be methylated for decades. Recent studies have found that mRNA is predominately m6A modified at stop codons and long internal exons, which are conserved between mouse and human. Emerging evidence suggests m6A plays an important role in regulating gene expression, alternative splicing patterns, downstream signalling (p53) as well as apoptosis. The regulation of m6A modifications in mRNA has been linked to disease, where fat mass and obesity-associated (FTO) has been has been reported to be a obesity risk gene. FTO is a m6A demethylase and polymorphisms that result in increased FTO expression are associated with increased body mass and risk of obesity.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Monoclonal
  • Clone number

    mAbcam 190886
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236160 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Nucleotide Array Use at an assay dependent concentration.

Target

  • Relevance

    N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
  • Alternative names

    • m6A antibody
    • N6-methyladenosine antibody

Images

  • ab190886 was tested in Nucleotide Array against N6-methyladenosine and unmodified adenosine. Six dilutions of each oligomer are printed on to the Array in triplicate and results are averaged before being plotted on to a graph. Results show strong binding to N6-methyladenosine, indicating that this antibody specifically recognises adenosine methylated at position N6.

    2Ome-m6A - N6-methyladenosine

    2Ome-m6A_unmod - unmodified adenosine

     

    This data was developed using the same antibody clone in a different buffer formulation containing PBS prepared using DEPC-treated water (ab190886).

References

ab236160 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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