Recombinant Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)
![Immunoprecipitation - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)](https://www.abcam.com/ps/products/208/ab208198/Images/ab208198-294013-anti-n6-n6-dimethyladenosine-m66a-antibody-epr-19831-44-immunoprecipitation.jpg "Immunoprecipitation - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR- 19831-44] to N6, N6-dimethyladenosine (m6,6A)
- Suitable for: IP, ELISA
Overview
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Product name
Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44]
See all N6, N6-dimethyladenosine (m6,6A) primary antibodies -
Description
Rabbit monoclonal [EPR- 19831-44] to N6, N6-dimethyladenosine (m6,6A) -
Host species
Rabbit -
Specificity
Has been developed to discriminate between the modified base N6, N6-dimethyladenosine (m6,6A) and the unmodified counterpart Adenosine (A). -
Tested applications
Suitable for: IP, ELISAmore details -
Species reactivity
Reacts with: Synthetic fragment -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IP: 5' Biotin-mN.mN.mN.mN.mN.[m6,6A]*.mN.mN.mN.mN.mN 3' * - phosphorothioate protection. ELISA: Biotinylated m6,6A-modified oligonucleotides.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR- 19831-44 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
0.2 µg of ab208198 was coated onto 50 µL Dynabeads® sheep-anti-rabbit IgG. |
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| ELISA |
Use a concentration of 0.005 - 4 µg/ml.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. 0.2 µg of ab208198 was coated onto 50 µL Dynabeads® sheep-anti-rabbit IgG. |
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ELISA
Use a concentration of 0.005 - 4 µg/ml. |
Target
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Relevance
N6,N6-dimethyladenosine(m6,6A) is an RNA modification found in rRNA in archaea, bacteria and eukaryotes. -
Alternative names
- Di-m6A antibody
- N6, N6 dimethyladenosine antibody
Images
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Immunoprecipitation - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)
The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.
ab208198 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.
Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.
After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.
ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.
Lane 1: Buffer only.
Lane 2: Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m6,6A]*.mN.mN.mN.mN.mN 3’
Lane 3: Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3’
N - equimolar mixture of (A/U/G/C)
m - 2’O methyl protection
* - phosphorothioate protectionBlocking buffer and concentration: 5% NFDM/TBST
Dilution buffer and concentration: TBST/0.1% Triton X-100/1 mM EDTA
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![ELISA - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)](https://www.abcam.com/ps/products/208/ab208198/Images/ab208198-303898-anti-n6-n6-dimethyladenosine-m66a-antibody-epr-19831-44-elisa.jpg)
ELISA - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)Biotinylated m6,6A (modified), m6A (modified) and A (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate.
Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m6,6A]*.mN.mN.mN.mN.mN 3’
Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m6A]*.mN.mN.mN.mN.mN 3’
Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3’
N - equimolar mixture of (A/U/G/C)
m - 2’O methyl protection
* - phosphorothioate protectionELISA was performed on 1.0 µg/ml of antigen using ab208198 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.
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![Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)](https://www.abcam.com/ps/products/208/ab208198/Images/ab208198-4-benefits-of-recombinant-antibodies.png)
Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab208198 has not yet been referenced specifically in any publications.
