Recombinant Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)
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Overview
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Product nameAnti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44]
See all N6, N6-dimethyladenosine (m6,6A) primary antibodies -
DescriptionRabbit monoclonal [EPR- 19831-44] to N6, N6-dimethyladenosine (m6,6A)
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Host speciesRabbit
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SpecificityHas been developed to discriminate between the modified base N6, N6-dimethyladenosine (m6,6A) and the unmodified counterpart Adenosine (A).
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Tested applicationsSuitable for: IP, ELISAmore details
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Species reactivityReacts with: Synthetic fragment
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Immunogen
Chemical/ Small Molecule corresponding to N6, N6-dimethyladenosine (m6,6A).
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Positive control
- IP: 5' Biotin-mN.mN.mN.mN.mN.[m6,6A]*.mN.mN.mN.mN.mN 3' * - phosphorothioate protection. ELISA: Biotinylated m6,6A-modified oligonucleotides.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR- 19831-44
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab208198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IP | Use at an assay dependent concentration. 0.2 µg of ab208198 was coated onto 50 µL Dynabeads® sheep-anti-rabbit IgG. |
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| ELISA | Use a concentration of 0.005 - 4 µg/ml. |
Target
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Alternative names
- Di-m6A antibody
- N6, N6 dimethyladenosine antibody
Images
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![Immunoprecipitation - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)](https://www.abcam.com/ps/products/208/ab208198/Images/ab208198-294013-208198ip.jpg)
Immunoprecipitation - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.
ab208198 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.
Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.
After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.
ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.
Lane 1: Buffer only.
Lane 2: Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m6,6A]*.mN.mN.mN.mN.mN 3’
Lane 3: Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3’
N - equimolar mixture of (A/U/G/C)
m - 2’O methyl protection
* - phosphorothioate protectionBlocking buffer and concentration: 5% NFDM/TBST
Dilution buffer and concentration: TBST/0.1% Triton X-100/1 mM EDTA
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![ELISA - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)](https://www.abcam.com/ps/products/208/ab208198/Images/ab208198-303898-m66A---03092018.jpg)
ELISA - Anti-N6, N6-dimethyladenosine (m6,6A) antibody [EPR- 19831-44] (ab208198)Biotinylated m6,6A (modified), m6A (modified) and A (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate.
Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m6,6A]*.mN.mN.mN.mN.mN 3’
Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m6A]*.mN.mN.mN.mN.mN 3’
Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[A]*.mN.mN.mN.mN.mN 3’
N - equimolar mixture of (A/U/G/C)
m - 2’O methyl protection
* - phosphorothioate protectionELISA was performed on 1.0 µg/ml of antigen using ab208198 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.
Datasheets and documents
References
ab208198 has not yet been referenced specifically in any publications.
