Anti-NAA38 antibody (ab236965)
Key features and details
- Rabbit polyclonal to NAA38
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NAA38 antibody -
Description
Rabbit polyclonal to NAA38 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human NAA38 aa 1 to the C-terminus. Full length chain of isoform 2.
Database link: Q9BRA0-2 -
Positive control
- WB: Mouse kidney tissue lysate. IHC-P: Human kidney tissue. ICC/IF: PC-3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab236965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000.
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IHC-P |
1/100 - 1/500.
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ICC/IF |
1/50 - 1/500.
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Notes |
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WB
1/500 - 1/5000. |
IHC-P
1/100 - 1/500. |
ICC/IF
1/50 - 1/500. |
Target
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Function
Component of the N-terminal acetyltransferase C (NatC) complex which may catalyze acetylation of N-terminal methionine residues. -
Sequence similarities
Belongs to the snRNP Sm proteins family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84316 Human
- Entrez Gene: 78304 Mouse
- SwissProt: Q9BRA0 Human
- SwissProt: Q9D2U5 Mouse
- Unigene: 565094 Human
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Alternative names
- LSM domain-containing protein 1 antibody
- lsmd1 antibody
- LSMD1_HUMAN antibody
see all
Images
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PC-3 (human prostate adenocarcinoma cell line) cells stained for NAA38 (Green) using ab236965 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
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Paraffin-embedded human kidney tissue stained for NAA38 using ab236965 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
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Anti-NAA38 antibody (ab236965) at 1/500 dilution + Mouse kidney tissue lysate
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab236965 has not yet been referenced specifically in any publications.