Key features and details
- Rabbit polyclonal to NAA38
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NAA38 antibody
DescriptionRabbit polyclonal to NAA38
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human NAA38 aa 2-173. Full length chain of isoform 2
MAVAVGVRAA PVLGLARALV LGLRGSQAAR WRGWGTAAPG SLWALCECPA GCRELWFRGR AAWAARSERL LHPAQDSDGE REDSAAERAR QQLEALLNKT MRIRMTDGRT LVGCFLCTDR DCNVILGSAQ EFLKPSDSFS AGEPRVLGLA MVPGHHIVSI EVQRESLTGP PYL
Database link: Q9BRA00-2
- WB: Mouse kidney tissue lysate. IHC-P: Human kidney tissue. ICC/IF: PC-3 cells.
This product was previously labelled as LSMD1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IHC-P||1/100 - 1/500.|
|ICC/IF||1/50 - 1/500.|
FunctionComponent of the N-terminal acetyltransferase C (NatC) complex which may catalyze acetylation of N-terminal methionine residues.
Sequence similaritiesBelongs to the snRNP Sm proteins family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- LSM domain-containing protein 1 antibody
- lsmd1 antibody
- LSMD1_HUMAN antibody
PC-3 (human prostate adenocarcinoma cell line) cells stained for NAA38 (Green) using ab236965 at 1/133 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
The cells were fixed in 4% formaldehyde, permeabilized tissue using 0.2% Triton X-100 and blocked in 10% normal goat serum. The cells were then incubated with the antibody overnight at 4°C. Counterstained with DAPI.
Paraffin-embedded human kidney tissue stained for NAA38 using ab236965 at 1/400 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
Anti-NAA38 antibody (ab236965) at 1/500 dilution + Mouse kidney tissue lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236965 has not yet been referenced specifically in any publications.