Overview

  • Product name

  • Description

    Rabbit polyclonal to NAB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Syrian hamster
  • Immunogen

    Synthetic peptide within Human NAB1 aa 460-487. The exact sequence is proprietary. (NP_005957.2).
    Database link: Q13506

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241305 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
  • Tissue specificity

    Isoform Short is found in a myeloid leukemia cell line.
  • Sequence similarities

    Belongs to the NAB family.
  • Domain

    The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
    The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EGR 1 binding protein 1 antibody
    • EGR-1-binding protein 1 antibody
    • EGR1 binding protein 1 antibody
    • NAB1 antibody
    • NAB1_HUMAN antibody
    • NGFI A binding protein 1 (EGR1 binding protein 1) antibody
    • NGFI A binding protein 1 antibody
    • NGFI-A-binding protein 1 antibody
    • Transcriptional regulatory protein p54 antibody
    see all

Images

  • NAB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) usind ab241305 at 6 µg/mg lysate. Western bot was performed on the immunoprecipitate using ab241305 at 0.4 µg/ml.

    Lane 1: ab241305 IP in HeLa whole cell lysate.
    Lane 2: Control IgG.

    Detection: Chemiluminescence with an exposure time of 10 seconds.

     

     

  • All lanes : Anti-NAB1 antibody (ab241305) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Exposure time: 3 minutes

References

ab241305 has not yet been referenced specifically in any publications.

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