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Epigenetics and Nuclear Signaling Transcription Other factors
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RecombinantRabMAb

Recombinant Anti-NAB1 antibody [EPR8227(2)] (ab150389)

  • Datasheet
  • SDS
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Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)
  • Anti-NAB1 antibody [EPR8227(2)] (ab150389)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8227(2)] to NAB1
  • Suitable for: WB
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-NAB1 antibody [EPR8227(2)]
    See all NAB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8227(2)] to NAB1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human NAB1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HepG2, HeLa, U87 MG and 293T cell lysates
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8227(2)
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Transcription factors

Associated products

  • Alternative Versions

    • Anti-NAB1 antibody [EPR8227(2)] - BSA and Azide free (ab248959)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa nuclear extract lysate (ab14655)
    • Hep G2 nuclear extract lysate (ab14660)
    • U-87 MG nuclear extract lysate (ab14903)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab150389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
    • Tissue specificity

      Isoform Short is found in a myeloid leukemia cell line.
    • Sequence similarities

      Belongs to the NAB family.
    • Domain

      The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
      The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q13506 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4664 Human
      • Entrez Gene: 17936 Mouse
      • Entrez Gene: 64824 Rat
      • Omim: 600800 Human
      • SwissProt: Q13506 Human
      • SwissProt: Q61122 Mouse
      • SwissProt: Q62722 Rat
      • Unigene: 723892 Human
      • Unigene: 25903 Mouse
      • Unigene: 10099 Rat
      see all
    • Alternative names

      • EGR 1 binding protein 1 antibody
      • EGR-1-binding protein 1 antibody
      • EGR1 binding protein 1 antibody
      • NAB1 antibody
      • NAB1_HUMAN antibody
      • NGFI A binding protein 1 (EGR1 binding protein 1) antibody
      • NGFI A binding protein 1 antibody
      • NGFI-A-binding protein 1 antibody
      • Transcriptional regulatory protein p54 antibody
      see all

    Images

    • Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)
      Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)
      All lanes : Anti-NAB1 antibody [EPR8227(2)] (ab150389) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : U87 MG cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 54 kDa

    • Anti-NAB1 antibody [EPR8227(2)] (ab150389)
      Anti-NAB1 antibody [EPR8227(2)] (ab150389)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab150389? Please let us know so that we can cite the reference in this datasheet.

    ab150389 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Western blot abreview for Anti-NAB1 antibody [EPR8227(2)]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    10 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Francis Mcmanus

    Verified customer

    Submitted Apr 18 2016

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