Recombinant
RabMAb

Anti-NAB1 antibody [EPR8227(2)] (ab150389)

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Overview

  • Product name
    Anti-NAB1 antibody [EPR8227(2)]
    See all NAB1 primary antibodies
  • Description
    Rabbit monoclonal [EPR8227(2)] to NAB1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NAB1 aa 100-200. The exact sequence is proprietary.

  • Positive control
    • HepG2, HeLa, U87 MG and 293T cell lysates
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function
      Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.
    • Tissue specificity
      Isoform Short is found in a myeloid leukemia cell line.
    • Sequence similarities
      Belongs to the NAB family.
    • Domain
      The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
      The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • EGR 1 binding protein 1 antibody
      • EGR-1-binding protein 1 antibody
      • EGR1 binding protein 1 antibody
      • NAB1 antibody
      • NAB1_HUMAN antibody
      • NGFI A binding protein 1 (EGR1 binding protein 1) antibody
      • NGFI A binding protein 1 antibody
      • NGFI-A-binding protein 1 antibody
      • Transcriptional regulatory protein p54 antibody
      see all

    Images

    • Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)
      Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)
      All lanes : Anti-NAB1 antibody [EPR8227(2)] (ab150389) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : U87 MG cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 54 kDa

    References

    ab150389 has not yet been referenced specifically in any publications.

    Publishing research using ab150389? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    1 Review or Q&A

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    Western blot abreview for Anti-NAB1 antibody [EPR8227(2)]

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    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    10 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
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    Username

    Francis Mcmanus

    Verified customer

    Submitted Apr 18 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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