Recombinant
RabMAb

Recombinant Anti-NAB1 antibody [EPR8227(2)] (ab150389)

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Overview

  • Product name

    Anti-NAB1 antibody [EPR8227(2)]
    See all NAB1 primary antibodies

  • Description

    Rabbit monoclonal [EPR8227(2)] to NAB1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human NAB1 aa 100-200. The exact sequence is proprietary.

  • Positive control

    • HepG2, HeLa, U87 MG and 293T cell lysates

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab150389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2.

    • Tissue specificity

      Isoform Short is found in a myeloid leukemia cell line.

    • Sequence similarities

      Belongs to the NAB family.

    • Domain

      The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
      The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • EGR 1 binding protein 1 antibody
      • EGR-1-binding protein 1 antibody
      • EGR1 binding protein 1 antibody
      • NAB1 antibody
      • NAB1_HUMAN antibody
      • NGFI A binding protein 1 (EGR1 binding protein 1) antibody
      • NGFI A binding protein 1 antibody
      • NGFI-A-binding protein 1 antibody
      • Transcriptional regulatory protein p54 antibody
      see all

    Images

    • Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)
      Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)
      All lanes : Anti-NAB1 antibody [EPR8227(2)] (ab150389) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : U87 MG cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution

      Predicted band size: 54 kDa

    References

    ab150389 has not yet been referenced specifically in any publications.

    Publishing research using ab150389? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Western blot abreview for Anti-NAB1 antibody [EPR8227(2)]

     Poor
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - nuclear (HEK293)
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    10 µg
    Specification
    HEK293
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Francis Mcmanus

    Verified customer

    Submitted Apr 18 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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