Recombinant Anti-NAB1 antibody [EPR8227(2)] (ab150389)
![Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)](https://www.abcam.com/ps/products/150/ab150389/Images/ab150389-156991-anti-nab1-antibody-epr82272-western-blot.jpg "Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8227(2)] to NAB1
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-NAB1 antibody [EPR8227(2)]
See all NAB1 primary antibodies -
Description
Rabbit monoclonal [EPR8227(2)] to NAB1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human NAB1 aa 100-200. The exact sequence is proprietary.
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Positive control
- HepG2, HeLa, U87 MG and 293T cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8227(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150389 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 54 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. -
Tissue specificity
Isoform Short is found in a myeloid leukemia cell line. -
Sequence similarities
Belongs to the NAB family. -
Domain
The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4664 Human
- Entrez Gene: 17936 Mouse
- Entrez Gene: 64824 Rat
- Omim: 600800 Human
- SwissProt: Q13506 Human
- SwissProt: Q61122 Mouse
- SwissProt: Q62722 Rat
- Unigene: 723892 Human
see all -
Alternative names
- EGR 1 binding protein 1 antibody
- EGR-1-binding protein 1 antibody
- EGR1 binding protein 1 antibody
see all
Images
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Western blot - Anti-NAB1 antibody [EPR8227(2)] (ab150389)All lanes : Anti-NAB1 antibody [EPR8227(2)] (ab150389) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : U87 MG cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 54 kDa -
![Anti-NAB1 antibody [EPR8227(2)] (ab150389)](https://www.abcam.com/ps/products/150/ab150389/Images/ab150389-2-benefits-of-recombinant-antibodies.png)
Anti-NAB1 antibody [EPR8227(2)] (ab150389)
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab150389 has not yet been referenced specifically in any publications.