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Rabbit polyclonal to Nab2
Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 475-525 of Human Nab2 (NP_005958.1)
Whole cell lysate from 293T and Jurkat cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate Note: pH 7 to 8.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
Use at 2-10 µg/mg of lysate.
Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
Widely expressed at low levels. Highly expressed in melanoma cell lines.
Belongs to the NAB family.
The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Nucleus. Isoform 2 is not localized to the nucleus.
Information by UniProt
EGR 1 binding protein 2 antibody
EGR-1-binding protein 2 antibody
EGR1 binding protein 2 antibody
Western blot - Anti-Nab2 antibody (ab112056)
All lanes : Anti-Nab2 antibody (ab112056) at 0.1 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg/ml Lane 2 : HeLa whole cell lysate at 15 µg/ml Lane 3 : HeLa whole cell lysate at 5 µg/ml Lane 4 : Jurkat whole cell lysate at 50 µg/ml Predicted band size: 57 kDa Exposure time: 3 minutes
Immunoprecipitation - Anti-Nab2 antibody (ab112056)
Immunoprecipitation of NAB2. Samples: 1 mg of whole cell lysate from 293T cells. NAB2 was detected with anti-NAB2 antibody NAB2 antibody (ab112056) at 1 µg/ml. Lane 1- NAB2 was also immunoprecipitated by rabbit anti-NAB2 antibody NAB2 antibody (ab112056) at 6 µg/mg lysate. Lane 2- NAB2 was immunoprecipitated by an rabbit anti-NAB2 antibody
that recognizes an upstream epitope. Lane 3- Rabbit IgG control.
Detection: Chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"