Key features and details
- Rabbit polyclonal to Nab2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nab2 antibody
See all Nab2 primary antibodies
DescriptionRabbit polyclonal to Nab2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide, corresponding to a region within amino acids 475-525 of Human Nab2 (NP_005958.1)
- Whole cell lysate from 293T and Jurkat cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab112056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 57 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
Tissue specificityWidely expressed at low levels. Highly expressed in melanoma cell lines.
Sequence similaritiesBelongs to the NAB family.
DomainThe NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Cellular localizationNucleus. Isoform 2 is not localized to the nucleus.
- Information by UniProt
- EGR 1 binding protein 2 antibody
- EGR-1-binding protein 2 antibody
- EGR1 binding protein 2 antibody
All lanes : Anti-Nab2 antibody (ab112056) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg/ml
Lane 2 : HeLa whole cell lysate at 15 µg/ml
Lane 3 : HeLa whole cell lysate at 5 µg/ml
Lane 4 : Jurkat whole cell lysate at 50 µg/ml
Predicted band size: 57 kDa
Exposure time: 3 minutes
Immunoprecipitation of NAB2. Samples: 1 mg of whole cell lysate from 293T cells. NAB2 was detected with anti-NAB2 antibody NAB2 antibody (ab112056) at 1 µg/ml. Lane 1- NAB2 was also immunoprecipitated by rabbit anti-NAB2 antibody NAB2 antibody (ab112056) at 6 µg/mg lysate. Lane 2- NAB2 was immunoprecipitated by an rabbit anti-NAB2 antibody that recognizes an upstream epitope. Lane 3- Rabbit IgG control. Detection: Chemiluminescence with exposure time of 10 seconds.
ab112056 has not yet been referenced specifically in any publications.