Overview

  • Product name

  • Description

    Rabbit polyclonal to Nab2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Nab2 (internal sequence). The exact sequence is proprietary.
    Database link: Q15742

  • Positive control

    • WB: Jurkat whole cell lysate. IP: Jurkat whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab229783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.
IP Use at an assay dependent dilution.

Target

  • Function

    Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
  • Tissue specificity

    Widely expressed at low levels. Highly expressed in melanoma cell lines.
  • Sequence similarities

    Belongs to the NAB family.
  • Domain

    The NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
    The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
  • Cellular localization

    Nucleus. Isoform 2 is not localized to the nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • EGR 1 binding protein 2 antibody
    • EGR-1-binding protein 2 antibody
    • EGR1 binding protein 2 antibody
    • MADER antibody
    • Melanoma associated delayed early response protein antibody
    • Melanoma-associated delayed early response protein antibody
    • MGC75085 antibody
    • Nab 2 antibody
    • nab2 antibody
    • NAB2_HUMAN antibody
    • NGFI A binding protein 2 (EGR1 binding protein 2) antibody
    • NGFI A binding protein 2 antibody
    • NGFI-A-binding protein 2 antibody
    • Protein MADER antibody
    see all

Images

  • Anti-Nab2 antibody (ab229783) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 57 kDa



    7.5% SDS-PAGE

  • Nab2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 µg ab229783. Western blot was performed from the immunoprecipitate using ab229783.

    Lane 1: Control IgG IP in Jurkat whole cell extract.

    Lane 2: ab229783 IP in Jurkat whole cell extract.

References

ab229783 has not yet been referenced specifically in any publications.

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