Key features and details
- Rabbit polyclonal to Nab2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Nab2 antibody
See all Nab2 primary antibodies
DescriptionRabbit polyclonal to Nab2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
Recombinant fragment within Human Nab2 (internal sequence). The exact sequence is proprietary.
Database link: Q15742
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
|IP||Use at an assay dependent dilution.|
FunctionActs as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. Isoform 2 lacks repression ability.
Tissue specificityWidely expressed at low levels. Highly expressed in melanoma cell lines.
Sequence similaritiesBelongs to the NAB family.
DomainThe NAB conserved domain 1 (NCD1) interacts with EGR1 inhibitory domain and mediates multimerization.
The NAB conserved domain 2 (NCD2) is necessary for transcriptional repression.
Cellular localizationNucleus. Isoform 2 is not localized to the nucleus.
- Information by UniProt
- EGR 1 binding protein 2 antibody
- EGR-1-binding protein 2 antibody
- EGR1 binding protein 2 antibody
Anti-Nab2 antibody (ab229783) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Nab2 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract with 5 µg ab229783. Western blot was performed from the immunoprecipitate using ab229783.
Lane 1: Control IgG IP in Jurkat whole cell extract.
Lane 2: ab229783 IP in Jurkat whole cell extract.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229783 has not yet been referenced specifically in any publications.