Overview

  • Product name

    Anti-NABC1 antibody [EPR15441(2)]
    See all NABC1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15441(2)] to NABC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NABC1 aa 500 to the C-terminus. The exact sequence is proprietary.
    Database link: O75363

  • Positive control

    • MCF7, SK-BR-3, HeLa and SW480 cell lysates; Human testis lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15441(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 62, 120 kDa (predicted molecular weight: 62 kDa).

Target

  • Tissue specificity

    Expressed in brain and prostate, and at lower levels in testis, intestine and colon. Overexpressed in most breast cancer cell lines and down-regulated in some colorectal tumors.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • AIBC 1 antibody
    • AIBC1 antibody
    • Amplified and overexpressed in breast cancer antibody
    • BCAS 1 antibody
    • BCAS1 antibody
    • BCAS1_HUMAN antibody
    • Breast carcinoma amplified sequence 1 antibody
    • Breast carcinoma-amplified sequence 1 antibody
    • Novel amplified in breast cancer 1 antibody
    see all

Images

  • All lanes : Anti-NABC1 antibody [EPR15441(2)] (ab201455) at 1/5000 dilution

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) cell lysate
    Lane 2 : SK-BR-3 (Human mammary gland adenocarcinoma cell line) cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate
    Lane 4 : SW480 (Human colorectal adenocarcinoma cell line) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 120,62 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature PMID: 14567997. Please see page 6. The band observed at 120 kDa may represent a homodimer of NABC1, this information is based on SwissProt (Section: Subunit Structure).

  • Anti-NABC1 antibody [EPR15441(2)] (ab201455) at 1/1000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 120,62 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The observed MW is consistent with what has been described in the literature PMID: 14567997. Please see page 6. The band observed at 120 kDa may represent a homodimer of NABC1, this information is based on SwissProt (Section: Subunit Structure).

References

ab201455 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201455.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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