• Product name

    Anti-Nac1 antibody
  • Description

    Rabbit polyclonal to Nac1
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Mouse Nac1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab30604.)

  • Positive control

    • This antibody gave a positive signal in the following lysates: F9 (Mouse embryonic carcinoma cell line) Whole Cell IOUD2 (Mouse embryonic stem cell, selected for Oct4 expression cell line) Whole Cell Brain (Rat) Tissue Lysate - normal tissue It also gave a positive result in FFPE human cerebral cortex sections.



Our Abpromise guarantee covers the use of ab29047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/250. Detects a band of approximately 57,60 kDa (predicted molecular weight: 57 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • Function

    Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
  • Tissue specificity

    Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
  • Sequence similarities

    Contains 1 BEN domain.
    Contains 1 BTB (POZ) domain.
  • Cellular localization

    Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • BEND8 antibody
    • BTB Domain Containing 14B antibody
    • BTB/POZ domain-containing protein 14B antibody
    • btbd14b antibody
    • FLJ37383 antibody
    • NAC-1 antibody
    • NAC1 antibody
    • Nacc1 antibody
    • NACC1_HUMAN antibody
    • Nucleus accumbens-associated protein 1 antibody
    see all


  • We are unsure as to the nature of the doublet. It is likely that the doublet is caused by the presence of processed and unprocessed forms of Nac1. Both bands are blocked by addition of the immunizing peptide (ab30604).
  • ab29047 stained in Hela cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab29047 at 1µg/ml and ab7291 (Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150120 (pseudo-colored red) and ab150081 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-Nac1 antibody (ab29047) at 1/250 dilution + Brain (Rat) Tissue Lysate - normal tissue at 10 µg

    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

  • ICC/IF image of ab29047 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab29047, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue).
  • IHC image of Nac1 staining in human cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab29047, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


This product has been referenced in:

  • Ju T  et al. Overexpression of NAC1 confers drug resistance via HOXA9 in colorectal carcinoma cells. Mol Med Rep 16:3194-3200 (2017). Read more (PubMed: 28713930) »
  • Han H  et al. Multilayered Control of Alternative Splicing Regulatory Networks by Transcription Factors. Mol Cell 65:539-553.e7 (2017). Read more (PubMed: 28157508) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Mouse Cell (Embryonic)
Yes - Triton X-100 (0.5%)
Blocking step
Serum as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 02 2015

Western blot
Human Cell lysate - whole cell (Melanoma)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Yuichi Ishikawa

Verified customer

Submitted Nov 12 2010

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