Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)
![Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)](https://www.abcam.com/ps/products/277/ab277093/Images/ab277093-413630-AntiNac1-antibody-PCRPNACC11A8-flowcyt-HeLa-cells.JPG "Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)")
Key features and details
- Mouse monoclonal [PCRP-NACC1-1A8] to Nac1
- Suitable for: Protein Array, Flow Cyt
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Nac1 antibody [PCRP-NACC1-1A8]
See all Nac1 primary antibodies -
Description
Mouse monoclonal [PCRP-NACC1-1A8] to Nac1 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Nac1 aa 1-527.
Database link: Q96RE7 -
Positive control
- Flow cyt: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99.9% PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate -
Clonality
Monoclonal -
Clone number
PCRP-NACC1-1A8 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab277093 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Protein Array | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1-2µg for 106 cells. |
Target
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Function
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines. -
Tissue specificity
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer. -
Sequence similarities
Contains 1 BEN domain.
Contains 1 BTB (POZ) domain. -
Cellular localization
Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 112939 Human
- Omim: 610672 Human
- SwissProt: Q96RE7 Human
- Unigene: 531614 Human
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Alternative names
- BEND8 antibody
- BTB Domain Containing 14B antibody
- BTB/POZ domain-containing protein 14B antibody
see all
Images
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Flow Cytometry - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Nac1 using ab277093 at 2 µg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
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![Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)](https://www.abcam.com/ps/products/277/ab277093/Images/ab277093-1-anti-nacc1-antibody-protein-array.jpg)
Protein Array - Anti-Nac1 antibody [PCRP-NACC1-1A8] (ab277093)Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277093.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab277093 has not yet been referenced specifically in any publications.
