Anti-NACA1 antibody (ab221950)
Key features and details
- Rabbit polyclonal to NACA1
- Suitable for: ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NACA1 antibody -
Description
Rabbit polyclonal to NACA1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Orangutan, Xenopus tropicalis -
Immunogen
Recombinant fragment corresponding to Human NACA1 aa 100-200.
Database link: Q13765 -
Positive control
- ICC/IF: SH-SY5Y cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Also reduces the inherent affinity of ribosomes for protein translocation sites in the ER membrane (M sites). May act as a specific coactivator for JUN, binding to DNA and stabilizing the interaction of JUN homodimers with target gene promoters. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the NAC-alpha family.
Contains 1 NAC-A/B (NAC-alpha/beta) domain.
Contains 1 UBA domain. -
Post-translational
modificationsPhosphorylation of Ser-43 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-159 by GSK3B may promote proteasome mediated degradation (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic. Also found in nucleus. - Information by UniProt
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Database links
- Entrez Gene: 513312 Cow
- Entrez Gene: 4666 Human
- Entrez Gene: 17938 Mouse
- Entrez Gene: 100172857 Orangutan
- Entrez Gene: 288770 Rat
- Entrez Gene: 443812 Xenopus laevis
- Entrez Gene: 100127557 Xenopus tropicalis
- Entrez Gene: 195823 Zebrafish
see all -
Alternative names
- Alpha NAC antibody
- Alpha-NAC antibody
- Hom s 2.02 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab221950 has not yet been referenced specifically in any publications.