Recombinant
RabMAb

Anti-NAD Synthetase antibody [EPR10611] (ab171942)

Overview

  • Product name
    Anti-NAD Synthetase antibody [EPR10611]
    See all NAD Synthetase primary antibodies
  • Description
    Rabbit monoclonal [EPR10611] to NAD Synthetase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NAD Synthetase aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q6IA69

  • Positive control
    • HeLa and 293T cell lysates; Human cervical carcinoma and testis tissues; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 79 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Relevance
      Nicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD)
    • Database links
    • Alternative names
      • Glutamine dependent NAD(+) synthetase antibody
      • NAD(+) synthase [glutamine hydrolyzing] antibody
      • NAD(+) synthetase 1 antibody
      • NADSYN1 antibody

    Images

    • All lanes : Anti-NAD Synthetase antibody [EPR10611] (ab171942) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 79 kDa

    • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling NAD Synthetase with ab171942 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NAD Synthetase with ab171942 at 1/50 dilution.

    • Immunofluorescent analysis of HeLa cells labeling NAD Synthetase with ab171942 at 1/50 dilution.

    • Flow cytometric analysis of permeabilized HeLa cells labeling NAD Synthetase with ab171942 at 1/100 dilution (red) ocompared with a Rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • James EL  et al. Replicatively senescent human fibroblasts reveal a distinct intracellular metabolic profile with alterations in NAD+ and nicotinamide metabolism. Sci Rep 6:38489 (2016). Read more (PubMed: 27924925) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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