Product nameAnti-NAD Synthetase antibody [EPR10611]
See all NAD Synthetase primary antibodies
DescriptionRabbit monoclonal [EPR10611] to NAD Synthetase
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NAD Synthetase aa 650 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q6IA69
- HeLa and 293T cell lysates; Human cervical carcinoma and testis tissues; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 79 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceNicotinamide adenine dinucleotide (NAD) is a coenzyme in metabolic redox reactions, a precursor for several cell signaling molecules, and a substrate for protein posttranslational modifications. NAD synthetase catalyzes the final step in the biosynthesis of NAD from nicotinic acid adenine dinucleotide (NaAD)
- Glutamine dependent NAD(+) synthetase antibody
- NAD(+) synthase [glutamine hydrolyzing] antibody
- NAD(+) synthetase 1 antibody
- NADSYN1 antibody
All lanes : Anti-NAD Synthetase antibody [EPR10611] (ab171942) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 79 kDa
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling NAD Synthetase with ab171942 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NAD Synthetase with ab171942 at 1/50 dilution.
Immunofluorescent analysis of HeLa cells labeling NAD Synthetase with ab171942 at 1/50 dilution.
Flow cytometric analysis of permeabilized HeLa cells labeling NAD Synthetase with ab171942 at 1/100 dilution (red) ocompared with a Rabbit IgG (negative) (green).