NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist (ab120099)
Key features and details
- Endogenous CB1 / TRPV1 agonist
- CAS Number: 199875-69-9
- Supplied in ethanol (5 mg/ml)
- Form / State: Liquid
- Source: Synthetic
Overview
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Product name
NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist -
Description
Endogenous CB1 / TRPV1 agonist -
Alternative names
- N-Arachidonyldopamine
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Biological description
Potent endogenous cannabinoid and vanilloid receptor agonist. (Ki values are 0.25 and 12 μM for CB1 and CB2, respectively). Potent agonist at TRPV1 (VR1) receptors (EC50 = ~50 nM). Active in vivo.
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CAS Number
199875-69-9 -
Chemical structure
Properties
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Chemical name
(5Z,8Z,11Z,14Z)-N-(3,5-dihydroxyphenethyl)icosa-5,8,11,14-tetraenamide -
Molecular weight
439.63 -
Molecular formula
C28H41NO3 -
PubChem identifier
5282105 -
Storage instructions
Store at -20°C (desiccating conditions). -
Solubility overview
Supplied in ethanol (5 mg/ml) -
Handling
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Oc1ccc(CCNC(=O)CCC/C=CC/C=CC/C=CC/C=CCCCCC)cc1O -
Source
Synthetic
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Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Vitamin D Receptor
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab120099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies |
Use at an assay dependent concentration.
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| Notes |
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Functional Studies
Use at an assay dependent concentration. |
Images
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Chemical Structure - NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist (ab120099)2D chemical structure image of ab120099, NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist
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Functional Studies - NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist (ab120099)ab15580 staining Ki67 in SK-N-SH cells treated with NADA (N-Arachidonyldopamine) (ab120099), by ICC/IF. Decrease in Ki67 expression correlates with increased concentration of NADA (N-Arachidonyldopamine), as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120099 (NADA (N-Arachidonyldopamine)) in ethanol, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15580 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (1)
ab120099 has been referenced in 1 publication.
- Ferreira SG et al. N-acyldopamines control striatal input terminals via novel ligand-gated cation channels. Neuropharmacology 56:676-83 (2009). PubMed: 19101577