NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist (ab120099)
- Datasheet
- References (1)
Overview
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Product name
NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist -
Description
Endogenous CB1 / TRPV1 agonist -
Alternative names
- N-Arachidonyldopamine
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Biological description
Potent endogenous cannabinoid and vanilloid receptor agonist. (Ki values are 0.25 and 12 μM for CB1 and CB2, respectively). Potent agonist at TRPV1 (VR1) receptors (EC50 = ~50 nM). Active in vivo. -
Purity
> 98% -
CAS Number
199875-69-9 -
Chemical structure
Properties
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Chemical name
(5Z,8Z,11Z,14Z)-N-(3,5-dihydroxyphenethyl)icosa-5,8,11,14-tetraenamide -
Molecular weight
439.63 -
Molecular formula
C28H41NO3 -
PubChem identifier
5282105 -
Storage instructions
Store at -20°C (desiccating conditions). -
Solubility overview
Supplied in ethanol (5 mg/ml) -
Handling
Providing storage is as stated on the product vial and the vial is kept tightly sealed, the product can be stored for up to 6 months. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Oc1ccc(CCNC(=O)CCC/C=CC/C=CC/C=CC/C=CCCCCC)cc1O -
Source
Synthetic
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Research areas
Applications
Our Abpromise guarantee covers the use of ab120099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
Images
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Functional Studies - NADA (N-Arachidonyldopamine), endogenous CB1 / TRPV1 agonist (ab120099)ab15580 staining Ki67 in SK-N-SH cells treated with NADA (N-Arachidonyldopamine) (ab120099), by ICC/IF. Decrease in Ki67 expression correlates with increased concentration of NADA (N-Arachidonyldopamine), as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120099 (NADA (N-Arachidonyldopamine)) in ethanol, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab15580 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
References
This product has been referenced in:
- O'Sullivan SE et al. Characterisation of the vasorelaxant properties of the novel endocannabinoid N-arachidonoyl-dopamine (NADA). Br J Pharmacol 141:803-12 (2004). Read more (PubMed: 14769783) »
- Huang SM et al. An endogenous capsaicin-like substance with high potency at recombinant and native vanilloid VR1 receptors. Proc Natl Acad Sci U S A 99:8400-5 (2002). Read more (PubMed: 12060783) »
- Bisogno T et al. N-acyl-dopamines: novel synthetic CB(1) cannabinoid-receptor ligands and inhibitors of anandamide inactivation with cannabimimetic activity in vitro and in vivo. Biochem J 351 Pt 3:817-24 (2000). Read more (PubMed: 11042139) »
