Overview

  • Product name

    Anti-NADase antibody
  • Description

    Rabbit polyclonal to NADase
  • Host species

    Rabbit
  • Specificity

    ab127942 reacts with NADase of Group A, C,and G origins
  • Tested applications

    Suitable for: WB, IP, Neutralisingmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Purified recombinant NADase of Group C hemolytic streptococci expressed in E. coli (Q5R2E3).

  • Positive control

    • Culture supernatants of hemolytic streptococci.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Whole serum
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab127942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 50 kDa.
IP Use at an assay dependent dilution.
Neutralising Use at an assay dependent dilution.

Target

  • Relevance

    NAD (nicotinamide adenine dinucleotide) hydrolyzing enzyme is one of the extracellular enzymes and toxins produced by hemolytic streptococci. Although its function as a toxin is largely unknown, it has been suggested to be related to pathogenicity of acute infection. NADase is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and the antibodies cross react each other. Upon infection of hemolytic streptococci, the antibody titer to the NADase increases similarly to anti SLO (Strreptolysin O) antibody.
  • Alternative names

    • nga antibody

Images

  • All lanes : Anti-NADase antibody (ab127942) at 1/2000 dilution

    Lane 1 : Culture supernatant of group A streptococcus
    Lane 2 : Culture supernatant of group C streptococcus
    Lane 3 : Culture supernatant only (negative control)

    Predicted band size: 50 kDa

References

This product has been referenced in:

  • Zhu L  et al. Intergenic Variable-Number Tandem-Repeat Polymorphism Upstream of rocA Alters Toxin Production and Enhances Virulence in Streptococcus pyogenes. Infect Immun 84:2086-93 (2016). Read more (PubMed: 27141081) »
  • Zhu L  et al. A molecular trigger for intercontinental epidemics of group A Streptococcus. J Clin Invest 125:3545-59 (2015). Read more (PubMed: 26258415) »
See all 5 Publications for this product

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