Key features and details
- Rabbit polyclonal to NADase
- Suitable for: WB, IP, Neutralising
- Reacts with: Species independent
- Isotype: IgG
Product nameAnti-NADase antibody
DescriptionRabbit polyclonal to NADase
Specificityab127942 reacts with NADase of Group A, C,and G origins
Tested applicationsSuitable for: WB, IP, Neutralisingmore details
Species reactivityReacts with: Species independent
Purified recombinant NADase of Group C hemolytic streptococci expressed in E. coli (Q5R2E3).
- Culture supernatants of hemolytic streptococci.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab127942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 50 kDa.|
|IP||Use at an assay dependent dilution.|
|Neutralising||Use at an assay dependent dilution.|
RelevanceNAD (nicotinamide adenine dinucleotide) hydrolyzing enzyme is one of the extracellular enzymes and toxins produced by hemolytic streptococci. Although its function as a toxin is largely unknown, it has been suggested to be related to pathogenicity of acute infection. NADase is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and the antibodies cross react each other. Upon infection of hemolytic streptococci, the antibody titer to the NADase increases similarly to anti SLO (Strreptolysin O) antibody.
- nga antibody
ab127942 has been referenced in 5 publications.
- Zhu L et al. Intergenic Variable-Number Tandem-Repeat Polymorphism Upstream of rocA Alters Toxin Production and Enhances Virulence in Streptococcus pyogenes. Infect Immun 84:2086-93 (2016). PubMed: 27141081
- Zhu L et al. A molecular trigger for intercontinental epidemics of group A Streptococcus. J Clin Invest 125:3545-59 (2015). PubMed: 26258415
- Minami M et al. Clindamycin-induced CovS-mediated regulation of the production of virulent exoproteins streptolysin O, NAD glycohydrolase, and streptokinase in Streptococcus pyogenes. Antimicrob Agents Chemother 54:98-102 (2010). PubMed: 19805566
- Kimoto H et al. Genetic and biochemical properties of streptococcal NAD-glycohydrolase inhibitor. J Biol Chem 281:9181-9 (2006). PubMed: 16380378
- Stevens DL et al. Molecular epidemiology of nga and NAD glycohydrolase/ADP-ribosyltransferase activity among Streptococcus pyogenes causing streptococcal toxic shock syndrome. J Infect Dis 182:1117-28 (2000). PubMed: 10979908