Key features and details
- Rabbit polyclonal to NaDC-3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NaDC-3 antibody
DescriptionRabbit polyclonal to NaDC-3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Zebrafish
- WB: Jurkat cell lysate. IHC: Human kidney tissue.
Previously labelled as SLC13A3.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab56017 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 61 kDa).|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
FunctionHigh-affinity sodium-dicarboxylate cotransporter that accepts a range of substrates with 4-5 carbon atoms. The stoichiometry is probably 3 Na(+) for 1 divalent succinate.
Tissue specificityExpression is highest in kidney. Detected in placenta, brain, liver and pancreas.
Sequence similaritiesBelongs to the SLC13A transporter (TC 2.A.47) family. NADC subfamily.
Cellular localizationCell membrane.
- Information by UniProt
- +dicarboxylate cotransporter 3 antibody
- H sapiens mRNA for hepatocyte nuclear factor 4 gamma antibody
- hNaDC3 antibody
Anti-NaDC-3 antibody (ab56017) at 2.5 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 61 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling NaDC-3 at 4-8µg/ml.
Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
ab56017 has been referenced in 1 publication.
- Ju W et al. Renal gene and protein expression signatures for prediction of kidney disease progression. Am J Pathol 174:2073-85 (2009). IHC-P ; Human, Mouse . PubMed: 19465643