NADH Assay Kit (Fluorometric, High Sensitivity) (ab234049)

Overview

  • Product name

    NADH Assay Kit (Fluorometric, High Sensitivity)
  • Detection method

    Fluorescent
  • Sample type

    Cell Lysate, Tissue Lysate
  • Species reactivity

    Reacts with: Other species, Mammals
  • Product overview

    NADH Assay Kit (Fluorometric, High Sensitivity) (ab234049) provides a highly sensitive method to detect low level NADH in a variety of samples including animal tissues, adherent or suspension cells and enzymatic reactions. The NADH Enzyme Mix specifically recognizes NADH (not NADPH) at levels less than 8nM in the enzyme reaction.

  • Notes

    NAD and NADH exist in all living organisms and are important for energy transformation and maintenance of the redox state of cells or tissues. The reduced form, NADH is a driving force for cells and plays a critical role in cell regulation and repair processes. It is of increasing interest to be able to measure low level of NADH in samples or in enzymatic reactions.

  • Platform

    Microplate reader

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 100 tests
    NADH Cycling Buffer 1 x 15ml
    NADH Cycling Enzyme Mix (Lyophilized) 1 vial
    NADH Extraction Buffer 1 x 50ml
    NADH Standard (Lyophilized) 1 vial
    PicoProbe™ (DMSO) 1 x 0.4ml

Images

  • Decomposition of NAD, but not NADH at 60oC for 30 minutes.

  • Measurement of NADH levels (pmol) in rat kidney (1.74 μg), muscle, (2.51 μg), heart (1.59 μg) and liver tissue lysates (29.5 μg).

Protocols

References

ab234049 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab234049.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up