Product nameAnti-NADH2 antibody [9E12-1B3] - N-terminal
DescriptionMouse monoclonal [9E12-1B3] to NADH2 - N-terminal
Tested applicationsSuitable for: WBmore details
Synthetic peptide corresponding to Human NADH2 (N terminal).
Database link: P03891
- WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn).
Western blot advice:
Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.36% HEPES, 0.87% Sodium chloride
Concentration information loading...
Purification notesPurified from hybridoma cell culture supernatant by biochemical fractionation from serum-free medium.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab219821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa).
Western blot using whole cell extracts is not recommended.
RelevanceNADH2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
Cellular localizationMitochondrion inner membrane; Multi-pass membrane protein.
- MTND2 antibody
- NADH dehydrogenase subunit 2 antibody
- NADH ubiquinone oxidoreductase chain 2 antibody
- ND2 antibody
All lanes : Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821) at 2 µg/ml
Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
ab219821 has not yet been referenced specifically in any publications.