Overview

  • Product name
    Anti-NADH2 antibody [9E12-1B3] - N-terminal
    See all NADH2 primary antibodies
  • Description
    Mouse monoclonal [9E12-1B3] to NADH2 - N-terminal
  • Host species
    Mouse
  • Tested applications
    Suitable for: WBmore details
  • Immunogen

    Synthetic peptide corresponding to Human NADH2 (N terminal).
    Database link: P03891

  • Positive control
    • WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn).
  • General notes

    Western blot advice:

    Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.

Properties

Applications

Our Abpromise guarantee covers the use of ab219821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa).

Western blot using whole cell extracts is not recommended.

Target

  • Relevance
    NADH2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
  • Cellular localization
    Mitochondrion inner membrane; Multi-pass membrane protein.
  • Alternative names
    • MTND2 antibody
    • NADH dehydrogenase subunit 2 antibody
    • NADH ubiquinone oxidoreductase chain 2 antibody
    • ND2 antibody

Images

  • All lanes : Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821) at 2 µg/ml

    Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
    Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-labeled Goat-anti-mouse IgG

    Developed using the ECL technique.

    Predicted band size: 39 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.

References

ab219821 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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