Key features and details
- Mouse monoclonal [9E12-1B3] to NADH2 - N-terminal
- Suitable for: WB
- Isotype: IgG2a
Product nameAnti-NADH2 antibody [9E12-1B3] - N-terminal
DescriptionMouse monoclonal [9E12-1B3] to NADH2 - N-terminal
Tested applicationsSuitable for: WBmore details
Synthetic peptide corresponding to Human NADH2 (N terminal).
Database link: P03891
- WB: Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn).
Western blot advice:
Hydrophobic intrinsic membrane proteins such as the core mtDNA-encoded proteins of the mitochondrial OXPHOS complexes tend to run faster in SDS-PAGE than predicted by their amino acid composition. This is likely due to incomplete unfolding of the protein and a more negative charge:mass ratio.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 0.36% HEPES, 0.87% Sodium chloride
Concentration information loading...
Purification notesPurified from hybridoma cell culture supernatant by biochemical fractionation from serum-free medium.
Light chain typekappa
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
Our Abpromise guarantee covers the use of ab219821 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 39 kDa).
Western blot using whole cell extracts is not recommended.
RelevanceNADH2 is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone
Cellular localizationMitochondrion inner membrane; Multi-pass membrane protein.
- MTND2 antibody
- NADH dehydrogenase subunit 2 antibody
- NADH ubiquinone oxidoreductase chain 2 antibody
- ND2 antibody
All lanes : Anti-NADH2 antibody [9E12-1B3] - N-terminal (ab219821) at 2 µg/ml
Lane 1 : Mitochondria from cultured normal control human dermal fibroblasts neonatal (HDFn)
Lane 2 : Mitochondria from HDFn cells depleted of mtDNA by long-term proliferation in the presence of ethidium bromide
Lysates/proteins at 10 µg per lane.
All lanes : HRP-labeled Goat-anti-mouse IgG
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?
Mitochondrial proteins solubilized in 2% SDS were separated by SDS-PAGE and then transferred to PVDF membranes in CAPS buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab219821 has not yet been referenced specifically in any publications.