Key features and details
- Rabbit polyclonal to NADK
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NADK antibody
See all NADK primary antibodies
DescriptionRabbit polyclonal to NADK
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human NADK aa 25-75. The exact sequence is proprietary.
Database link: O95544
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysate. IP: HeLa cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to NADK immobilized on solid support.
Our Abpromise guarantee covers the use of ab241424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityWidely expressed but not detected in skeletal muscle.
Sequence similaritiesBelongs to the NAD kinase family.
- Information by UniProt
- dJ283E3.1 antibody
- FLJ13052 antibody
- FLJ37724 antibody
All lanes : Anti-NADK antibody (ab241424) at 1 µg/ml
Lanes 1 & 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
NADK was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241424 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241424 at 1 µg/ml.
Lane 1: ab241424 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241424 has not yet been referenced specifically in any publications.