Overview

  • Product name

    NADPH Assay Kit (Colorimetric)
    See all NADPH kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant
  • Assay type

    Quantitative
  • Sensitivity

    > 3 µM
  • Range

    3.13 µM - 100 µM
  • Assay time

    2h 00m
  • Product overview

    Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+. NAD forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. The traditional NAD/NADH and NADP/NADPH assays are based on monitoring the changes in NADH or NADPH absorption at 340 nm. The short UV wavelength of NAD/NADH and NADP/NADPH assays makes the traditional methods suffer low sensitivity and high interference.

    Abcam’s Colorimetric NADPH Assay Kit (ab186031) provides a convenient method for detecting NADPH. The NADPH probe is a chromogenic sensor that has its maximum absorbance at 460 nm upon NADPH reduction. The absorbance maximum increases to ~ 635 nm if the enhancer is added to the assay system. The absorption of the NADPH probe is directly proportional to the concentration of NADPH in the solution. The Colorimetric NADPH Assay Kit provides a sensitive assay to detect as little as 3 µM NADPH in a 100 µL assay volume.

  • Platform

    Microplate reader

Properties

Images

  • NADPH dose response was measured with the Colorimetric NADPH Assay Kit in a 96-well white/clear bottom plate using a microplate reader. As low as 3 µM of NAPDH can be detected with 30min incubation (n=3) with absorbance measurement at 460nm.

    The absorbance in blank wells (with the PBS buffer only) is used as a control, and is subtracted from the values for those wells with the NADPH reactions.

     

     

     

     

Protocols

References

ab186031 has not yet been referenced specifically in any publications.

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