Overview

  • Product name
    Anti-NADPH oxidase 4 antibody
    See all NADPH oxidase 4 primary antibodies
  • Description
    Rabbit polyclonal to NADPH oxidase 4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 252-564 of Human NOX4 (UniProt ID: Q9NPH5).

  • Positive control
    • 293T, A431, Jurkat and Raji whole cell lysates; A431 cells; Human colon carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab154244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 67 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.
  • Tissue specificity
    Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
  • Sequence similarities
    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • Developmental stage
    Expressed in fetal kidney and fetal liver.
  • Post-translational
    modifications
    Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
  • Cellular localization
    Endoplasmic reticulum membrane. Cell membrane. Cell junction > focal adhesion. Nucleus. May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Kidney oxidase 1 antibody
    • Kidney oxidase-1 antibody
    • Kidney superoxide producing NADPH oxidase antibody
    • Kidney superoxide-producing NADPH oxidase antibody
    • KOX 1 antibody
    • KOX antibody
    • Kox-1 antibody
    • KOX1 antibody
    • NADPH antibody
    • NADPH oxidase 4 antibody
    • Nox4 antibody
    • NOX4_HUMAN antibody
    • Renal NAD(P)H oxidase antibody
    • Renal NAD(P)H-oxidase antibody
    • RENOX antibody
    see all

Images

  • All lanes : Anti-NADPH oxidase 4 antibody (ab154244) at 1/1000 dilution

    Lane 1 : 293T whole cell extract
    Lane 2 : HeLa whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 67 kDa

  • ab154244 staining NADPH oxidase 4 in A431 cells fixed in ice-cold MeOH for 5 minutes. Samples were incubated with primary antibody at 1:500 dilution. Hoechst 33342 staining was used (Blue).

  • ab154244 staining  NADPH oxidase 4 in paraffin embedded rat kidney tissue by Immunohistochemistry  (IHC-P- paraffin embedded sections). Samples were incubated with primary antibody at 1:500 dilution.

  • Anti-NADPH oxidase 4 antibody (ab154244) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 67 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol fixed A431 cells labeling NOX4 with ab154244 at 1/500. Lower panel shows costaining with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Human colon carcinoma tissue labeling NOX4 with ab154244 at 1/250.

References

This product has been referenced in:
  • de Jesus DS  et al. Nox1/Ref-1-mediated activation of CREB promotes Gremlin1-driven endothelial cell proliferation and migration. Redox Biol 22:101138 (2019). Read more (PubMed: 30802716) »
  • Geng Z  et al. FNDC5 attenuates obesity-induced cardiac hypertrophy by inactivating JAK2/STAT3-associated inflammation and oxidative stress. J Transl Med 17:107 (2019). Read more (PubMed: 30940158) »
See all 6 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Denaturing (10% acrylamide gel)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

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Verified customer

Submitted Aug 02 2018

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