Western blot abreview for Anti-NADPH oxidase 4 antibody

Inconclusive
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Non-reduced Denaturing (10% acrylamide gel)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk
Dilution
1/1000

Secondary antibody

Name

Detection

Detection method
Super Signal
Negative control
Your samples
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: None kDa Non-specific: 100, 150 kDa
Positive control
Your samples

Additional data

Additional Notes
Did not see a band at the correct molecular weight for Nox4 using C57BL/6 mice Liver samples. However, Nox2 antibodies worked very well with this strain of mice.
Abcam response
Thank you for submitting your feedback on use of ab154244 in mouse. We have not validated in mouse samples and will continue to gather data on use in this species.

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Submitted Aug 02 2018

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