Western blot abreview for Anti-NADPH oxidase 4 antibody [UOTR1B492]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount
25 µg
Specification
Glioblastoma primary cells
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk 1% in TBS Tween 0.1%

Secondary antibody

Name
Non-Abcam antibody was used: Bio-Rad Goat Anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 62 kDa

Additional data

Additional Notes
Could be used at 1/2000

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Submitted May 08 2013

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