Immunocytochemistry/ Immunofluorescence abreview for Anti-NADPH oxidase 4 antibody [UOTR1B493]

Below Average
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - 0.1% Triton X100
Specification
HEK293T
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Donkey serum, 0.1% Triton X100
Dilution
1/200

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: Invitrogen
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

Additional data

Abcam response
Our Scientific Support team is currently in contact with this researcher, and we will resolve the issue in accordance with our Abpromise.

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Submitted May 17 2017

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