Western blot abreview for Anti-NADPH oxidase 4 antibody [UOTR1B493]

Good
Abreviews
Application
Western blot
Sample
Pig Tissue lysate - whole (Kidney)
Gel Running Conditions
Reduced Denaturing
Loading amount
35 µg
Specification
Kidney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: ~67 kDa

Additional data

Additional Notes
Lane 1 and 2: Pig kidney samples

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Submitted Nov 29 2017

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