Western blot abreview for Anti-NADPH oxidase 4 antibody [UOTR1B493]

Inconclusive
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (RPE1 cells)
Gel Running Conditions
Reduced Denaturing (Bis Tris 4-12%)
Loading amount
70000 cells
Treatment
esiRNA for 72h
Specification
RPE1 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000

Secondary antibody

Dilution
1/20000
Name
Non-Abcam antibody was used: LI-COR 92632213
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye® 800CW

Detection

Detection method
LI-COR
Negative control
esiRNA of Nox4
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 0 kDa Non-specific: 55, 60, 65, 27 kDa
Positive control
esiControl and untreated cells

Additional data

Additional Notes
esiRNa samples 72h after transfection Line1: esiControl Line2: esiRNA Nox4 Line3: untreated cells

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Submitted Feb 28 2018

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