Western blot abreview for Anti-NADPH oxidase 4 antibody [UOTR1B493]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (RPE)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
RPE
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
16 hour(s) and 0 minute(s)
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 64 kDa Non-specific: 59 kDa

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Submitted Apr 18 2019

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