Overview

Description

  • Nature
    Synthetic
  • Amino Acid Sequence

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab179799 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-NADPH oxidase 4 antibody [UOTR1B492] (ab109225)

    • Form
      Liquid
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    General Info

    • Alternative names
      • Kidney oxidase 1
      • Kidney oxidase-1
      • Kidney superoxide producing NADPH oxidase
      • Kidney superoxide-producing NADPH oxidase
      • KOX
      • KOX 1
      • Kox-1
      • KOX1
      • NADPH
      • NADPH oxidase 4
      • Nox4
      • NOX4_HUMAN
      • Renal NAD(P)H oxidase
      • Renal NAD(P)H-oxidase
      • RENOX
      see all
    • Function
      Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.
    • Tissue specificity
      Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
    • Sequence similarities
      Contains 1 FAD-binding FR-type domain.
      Contains 1 ferric oxidoreductase domain.
    • Developmental stage
      Expressed in fetal kidney and fetal liver.
    • Post-translational
      modifications
      Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
    • Cellular localization
      Endoplasmic reticulum membrane. Cell membrane. Cell junction > focal adhesion. Nucleus. May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
    • Information by UniProt

    References

    ab179799 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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