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Abcam's NADP/NADPH Assay Kit (Fluorometric) (ab176724) provides a convenient method for sensitive detection of NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme recycling reaction that significantly increases detection sensitivity. In addition, this assay has very low background since it is run in the red visible range that considerably reduces the sample interference. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 530-570/590-600 nm (maximum Ex/Em = 540/590 nm) or an absorbance microplate reader at ~576 nm. This also provides NADP, NADPH extraction buffer, and cell lysis buffer.
Nicotinamide adenine dinucleotide (NAD+) and nicotinamide adenine dinucleotide phosphate (NADP+) are two important cofactors found in cells. NADH is the reduced form of NAD+, and NAD+ is the oxidized form of NADH. NAD forms NADP with the addition of a phosphate group to the 2' position of the adenyl nucleotide through an ester linkage. NADP is used in anabolic biological reactions, such as fatty acid and nucleic acid synthesis, which require NADPH as a reducing agent. In chloroplasts, NADP is an oxidizing agent important in the preliminary reactions of photosynthesis. The NADPH produced by photosynthesis is used as reducing power for the biosynthetic reactions in the Calvin cycle of photosynthesis.
|NADP Extraction Solution||1 x 10ml|
|NADP/NADPH Control Solution||1 x 10ml|
|NADP/NADPH Lysis Buffer||1 x 10ml|
|NADP/NADPH Recycling Enzyme Mixture||2 vials|
|NADPH Extraction Solution||1 x 10ml|
|NADPH Sensor Buffer||1 x 20ml|
|NADPH Standard||1 vial|
Quantity of NADP, NADPH and total (NADP+NADPH) in U937 cells (duplicates; +/- SD). 0.5 x 107 cells were lysed in 1 mL of lysis buffer.
Quantity of NADP, NADPH and total (NADP+NADPH) in RAW 264.7 cells (duplicates; +/- SD). 0.5 x 107 cells were lysed in 1 mL lysis buffer.
Standard curves with background signal subtracted (duplicates; +/- SD).
ab176724 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"