Anti-NAF1 antibody (ab122323)

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Overview

  • Product name

    Anti-NAF1 antibody
    See all NAF1 primary antibodies

  • Description

    Rabbit polyclonal to NAF1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    antigen: EIFGPVAHPF YVLRFNSSDH IESKGIKIKE TMYFAPSMKD FTQYIFTEKL KQDKGSDASW KNDQEPPPEA LDFSDDEKEK EAKQRKKSQI QGRK, corresponding to amino acids 242-335 of Human NAF1 (Q96HR8).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human esophagus tissue and human cell line U-2 OS

Properties

Applications

Our Abpromise guarantee covers the use of ab122323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Function

    RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.

  • Sequence similarities

    Belongs to the NAF1 family.

  • Cellular localization

    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus.
  • Information by UniProt

  • Database links

    • Alternative names

      • H/ACA ribonucleoprotein complex non-core subunit NAF1 antibody
      • hNAF1 antibody
      • Naf1 antibody
      • NAF1_HUMAN antibody
      • Nuclear assembly factor 1 homolog antibody
      • Nuclear assembly factor 1 ribonucleoprotein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAF1 antibody (ab122323)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAF1 antibody (ab122323)
      Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human esophagus with ab122323 at 1/100 dilution showing strong nuclear and cytoplasmic positivity in squamous epithelial cells
    • Immunocytochemistry/ Immunofluorescence - Anti-NAF1 antibody (ab122323)
      Immunocytochemistry/ Immunofluorescence - Anti-NAF1 antibody (ab122323)
      Immunofluorescent staining of human cell line U-2 OS (Fixation/Permeabilization: PFA/Triton X-100) with ab122323 at 4ug/ml shows positivity in cytoplasm, centrosome & cell junctions. Antibody staining is shown in green.

    References

    ab122323 has not yet been referenced specifically in any publications.

    Publishing research using ab122323? Please let us know so that we can cite the reference in this datasheet.

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