Anti-NAF1 antibody (ab122323)
Key features and details
- Rabbit polyclonal to NAF1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NAF1 antibody
See all NAF1 primary antibodies -
Description
Rabbit polyclonal to NAF1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen: EIFGPVAHPF YVLRFNSSDH IESKGIKIKE TMYFAPSMKD FTQYIFTEKL KQDKGSDASW KNDQEPPPEA LDFSDDEKEK EAKQRKKSQI QGRK, corresponding to amino acids 242-335 of Human NAF1 (Q96HR8).
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Positive control
- IHC: Human esophagus tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab122323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4. -
Sequence similarities
Belongs to the NAF1 family. -
Cellular localization
Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 92345 Human
- SwissProt: Q96HR8 Human
- Unigene: 129095 Human
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Alternative names
- H/ACA ribonucleoprotein complex non-core subunit NAF1 antibody
- hNAF1 antibody
- Naf1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAF1 antibody (ab122323)Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human esophagus with ab122323 at 1/100 dilution showing strong nuclear and cytoplasmic positivity in squamous epithelial cells
Protocols
Datasheets and documents
References (0)
ab122323 has not yet been referenced specifically in any publications.
