Overview

  • Product name
    Anti-NAF1 antibody
    See all NAF1 primary antibodies
  • Description
    Rabbit polyclonal to NAF1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen: EIFGPVAHPF YVLRFNSSDH IESKGIKIKE TMYFAPSMKD FTQYIFTEKL KQDKGSDASW KNDQEPPPEA LDFSDDEKEK EAKQRKKSQI QGRK, corresponding to amino acids 242-335 of Human NAF1 (Q96HR8).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Human esophagus tissue and human cell line U-2 OS

Properties

Applications

Our Abpromise guarantee covers the use of ab122323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.

Target

  • Function
    RNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
  • Sequence similarities
    Belongs to the NAF1 family.
  • Cellular localization
    Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus.
  • Information by UniProt
  • Database links
    • Alternative names
      • H/ACA ribonucleoprotein complex non-core subunit NAF1 antibody
      • hNAF1 antibody
      • Naf1 antibody
      • NAF1_HUMAN antibody
      • Nuclear assembly factor 1 homolog antibody
      • Nuclear assembly factor 1 ribonucleoprotein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAF1 antibody (ab122323)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAF1 antibody (ab122323)
      Immunohistochemical staining(Formalin/PFA-fixed paraffin-embedded sections) of human esophagus with ab122323 at 1/100 dilution showing strong nuclear and cytoplasmic positivity in squamous epithelial cells
    • Immunocytochemistry/ Immunofluorescence - Anti-NAF1 antibody (ab122323)
      Immunocytochemistry/ Immunofluorescence - Anti-NAF1 antibody (ab122323)
      Immunofluorescent staining of human cell line U-2 OS (Fixation/Permeabilization: PFA/Triton X-100) with ab122323 at 4ug/ml shows positivity in cytoplasm, centrosome & cell junctions. Antibody staining is shown in green.

    References

    ab122323 has not yet been referenced specifically in any publications.

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